摘要
目的对邻苯三酚自氧化法测定血浆超氧化物歧化酶（SOD）的方法学进行评价,并探索其临床应用价值。方法根据美国临床和实验室标准协会（CLSI）的评价方法,应用邻苯三酚自氧化法检测血浆SOD,评价其精密度、准确度、线性和回收率;随机选取2014年健康体检者42例作为对照组,2014年3-12月我院住院氧化应激反应相关疾病患者1580例作为疾病组,测定血浆SOD的活性水平,进行临床应用评价。结果用邻苯三酚自氧化法测定SOD精密度试验中批内精密度为1.57%和4.8%,批间精密度为2.17和2.21%;准确性试验相对偏倚为：1.6%和2.4%;线性试验在0-250 U/ml内R2为0.9978;回收率为96.2%-103.4%;SOD在各疾病组与健康对照组差异均具有显著性（P〈0.01）。结论邻苯三酚自氧化法测定SOD方法学评价符合要求,其精密度高、准确性好,线性范围宽,具有较高的临床应用价值。
Objective To evaluate the methodology of determination of plasma superoxide dismutase（ SOD） by means of pyrogallol autoxidation and explore its clinical application. Methods Based on the USA Clinical and Laboratory Standards Institute（ CLSI）,plasma SOD was determined using pyrogallol autoxidation to evaluate its precision,accuracy,linearity and recovery rate. 42 healthy checkup people in 2014 were selected as a control group and 1580 cases of inpatients from March to December in 2014 who had related disease with oxidative stress reaction were chosen as a disease group. Their level activity of plasma SOD was determined. These inpatients were divided into 6 disease groups in terms of liver,renal,digestive system,respiratory system,cardiovascular,and diabetes diseases. Results By the test of determining the precision of SOD with pyrogallol autoxidation,the within-run precision were 1. 57% and 4. 8% and the between-run precision were 2. 17% and 2. 21%. The relative biases were 1. 6% and 2. 4% in the accuracy test. R2 was 0. 9978 within 0 - 250 U / ml in the linear test. Recovery rates were 96. 2% - 103. 4%. There were significant differences of SOD between each disease group and healthy control group（ P〈 0. 01）. Conclusion The methodological evaluation of determining SOD with pyrogallol autoxidation meets the requirement for its high precision,good accuracy and wide linear range. The method has high value in clinical application.
出处
《淮海医药》
CAS
2015年第3期227-229,共3页
Journal of Huaihai Medicine
