摘要
目的:研究索拉菲尼对结肠癌Ht-29细胞生长抑制作用,观察其对bax、bcl-2mRNA表达的影响。方法:MTT法测定索拉菲尼对Ht-29细胞株生长抑制率,Hoechst荧光染色观察细胞凋亡及形态的变化,Realtime-PCR检测bcl-2、bax mRNA表达情况。结果:索拉菲尼对Ht-29细胞株有生长抑制作用及促凋亡作用,拉菲尼处理后的Ht-29细胞中bcl-2 mRNA表达均较空白对照组降低,baxmRNA表达逐渐增强。结论:索拉菲尼可明显抑制荷Ht-29细胞增殖并诱导其凋亡,其作用机制可能与调控癌基因及凋亡基因表达有关。
OBJECTIVE： To investigate the effects of sorafenib on the proliferation and apoptosis of colon cancer Ht-29 cells. METHODS： Growth inhibition of Ht-29 cells by different concentrations of sorafenibwas evaluated by MTT assay. The effects of sorafenib on the apoptotic morphological changes were analyzed by Hoechst 33258 staining.The expressions of bcl-2, bax of Ht-29 cells treated with different concentrations of sorafenibwas were detected by Realtime-PCR. RESULTS： Sorafenib could had significantly inhibitory action on growth and apoptosis of Ht-29 cells, bcl-2 expressions of Ht-29 cells treated with different concentrations of sorafenib were significantly lower than the control group, while the bax expressions significantly upregulated, and the effects appeared to be dose-related （P〈0.05）. CONCLUSION： Sorafenib could inhibit proliferation and induce apoptosis of colon cancer Ht-29 cells and has a great correlativity to the expressions of related oncogene and apoptosis- related genes.
出处
《中国医学装备》
2014年第B12期114-114,共1页
China Medical Equipment
