摘要
为选育优质花椰菜新品种,指导种质资源引进和利用,本研究采用简单重复序(simple sequence repeat,SSR)标记和相关序列扩增多态性(sequence-related amplified polymorphism,SRAP)标记对38份花椰菜自交系进行了遗传多样性分析,分别从48对SSR引物、48对SRAP引物中各筛选出4对有效引物。4对SSR引物扩增的总条带数为47个,多态性条带为39个,平均多态性比率达83.0%;4对SRAP引物扩增的总条带数为86个,多态性条带为51个,平均多态性比率为59.3%,该结果显示花椰菜自交系间具有较丰富的遗传多样性。UPGMA聚类分析揭示了花椰菜自交系的熟期与其遗传差异相关。
The genetic diversity analysis of 38 cauliflower inbred lines was useful for cauliflower breeding,introduction and utilization of cauliflower germplasm resources by simple sequence repeat（SSR） and sequencerelated amplificated polymorphism（SRAP） markers. Four SSR primers and four SRAP primers with polymorphism and repeatability were selected from 48 SSR primers and 48 SRAP primers. A total of 47 bands were generated by four SSR primers, including 39 polymorphic bands, with an average of 83.0% polymorphism. Four SRAP primers had totally amplified 86 bands with an average of 59.3% polymorphism, of which 51 were polymorphic. The results showed that 38 cauliflower inbred lines were rich in genetic diversity. Cluster analysis with UPGMA method revealed that maturity of the inbred lines were associated with genetic differences.
出处
《分子植物育种》
CAS
CSCD
北大核心
2015年第3期605-614,共10页
Molecular Plant Breeding
基金
温州科技计划项目(N20100006)资助
