摘要
目的 :探讨嗅三针对阿茨海默病(AD)大鼠学习记忆能力及海马组织钙依赖性蛋白激酶Ⅱ(CaMKⅡ)的影响。方法:成年SD雄性大鼠50只,随机分为正常对照组、AD模型组、AD嗅神经切断模型组、嗅三针组和嗅三针对照组,每组10只。制作AD大鼠模型和AD嗅神经切断大鼠模型,通过嗅三针治疗,测试大鼠水迷宫学习记忆能力并采用ELISA法测定海马组织CaMKⅡ含量。结果:水迷宫定位航行试验显示:平均逃避潜伏期和平均游泳路程比较,正常对照组和嗅三针组均明显短于AD模型组(P<0.01),嗅三针对照组短于AD模型组(P<0.05),嗅三针组短于嗅三针对照组(P<0.05),AD模型组与AD嗅神经切断模型组比较,其差异无统计学意义(P>0.05)。海马组织CaMKⅡ含量比较:正常对照组、嗅三针组和嗅三针对照组均明显低于AD模型组(P<0.01),嗅三针组低于嗅三针对照组(P<0.05),AD模型组与AD嗅神经切断模型组比较,差异无统计学意义(P>0.05)。结论:嗅三针能够显著增强AD大鼠学习记忆功能、并且能提高海马组织CaMKⅡ含量表达,其治疗效应的发挥依赖于嗅觉传导通路的完整性。
Objective：To explore the effect of smell three-needle therapy on learning and memory ability and CaMKⅡ contents in hippocampus of rats with Alzheimer＇s disease（AD）. Methods：50 adult male SD rats were randomly divided into 5 groups：normal control group,AD model group,AD model with severed olfactory nerve group,smell three-needle therapy group and control group for smell three-needle therapy. Each group had 10 rats. AD models and AD models with severed olfactory nerve were established. Morris maze tests were conducted for evaluating the learning and memory abilities. The CaMKⅡ contents of hippocampus were detected by ELISA. Results：For the comparison of average escape latency and travel distance,the nor mal control group and smell three-needle therapy group was significantly shorter than the AD group（P 〈0.01）. The control group for smell three-needle therapy was shorter than the AD group（P 〈0.05）. The smell three-needle therapy group was shorter than the control group for smell three-needle therapy（P 〈0.05）. No significant differences were found between AD model group and AD model with severed olfactory nerve group（P 〈0.05）.For the comparison of CaMK Ⅱ contents,the AD model group were much more higher than normal control group,smell three-needle therapy group and control group for smell three-needle therapy（P 〈0.01）. The smell three-needle therapy group was lower than control group for smell three-needle therapy group（P 〈0.05）;No significant differences were found between AD model group and AD model with severed olfactory nerve group（P 〈0.05）. Conclusion：The smell three-needle therapy can dramatically increased the learning and memory abilities and decreased CaMKⅡ contents in hippocampus of rats with Alzheimer＇s disease. Its therapeutical effects depended on the entirety of olfactory pathway.
出处
《山东中医药大学学报》
2015年第2期166-168,共3页
Journal of Shandong University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(编号:81273859)
关键词
阿茨海默病
嗅觉
海马
学习记忆
钙依赖性蛋白激酶Ⅱ
嗅三针
大鼠
Alzheimer＇s disease（AD）
olfactory
hippocampus
learning and memory
CaMKⅡ
smell three-needle therapy
rat
