摘要
目的:探讨长期有氧训练对心力衰竭(HF)大鼠心脏重塑(包括结构性重塑和功能性重塑)及运动耐力的影响。方法:8周龄清洁级雄性Wistar大鼠结扎冠状动脉建立HF模型,4周后随机分为假手术对照组(Sham组)、假手术运动组(Sham+E组)、心衰对照组(HF组)和心衰运动组(HF+E组)。Sham+E组和HF+E组进行10周有氧训练(跑台),Sham组和HF组保持安静状态。实验前后利用超声心动术检测心脏结构参数包括左室舒张期内径(LVIDd)、左室收缩期内径(LVIDs)、左室舒张期前壁厚度(LVAWDd)、左室收缩期前壁厚度(LVAWDs)、左室舒张期后壁厚度(LVPWDd)、左室收缩期后壁厚度(LVPWDs)以及心功能参数包括缩短分数(FS)、左室射血分数(LVEF)和心率(HR);利用跑台递增负荷力竭运动实验测定大鼠的运动耐力参数包括力竭时间、力竭距离和最大跑速。实验后取心脏称量体重(BW)、左室重量(LVW)、右室重量(RVW)并计算左室质量指数(LVMI),利用Masson染色法进行组织病理学观察并获得心肌胶原容积分数(CVF)。结果:1)与Sham组比较,HF组最大跑速、力竭距离、力竭时间、BW、LVIDd、FS和LVEF降低(P<0.01),LVW、LVMI、LVAWDd、LVAWDs、LVPWDd、LVPWDs和CVF升高(P<0.01);2)与HF组比较,HF+E组最大跑速、力竭距离和力竭时间、LVW、LVMI、LVIDd、FS和LVEF升高(P<0.01),HR和CVF降低(P<0.01)。结论:1)HF后心脏出现结构与功能重塑,运动能力下降;2)10周有氧跑台训练逆转了HF大鼠心脏重塑,运动耐力提高。
Objective：To investigate the effect of 10 weeks aerobic training on cadiac remodeling （in-cluding structure remodeling and function remodeling）as well as exercise tolerance of heart failure （HF）rats.Methods：HF model was established through ligating coronary artery in clean grade Wistar rats of 8 weeks.After 4 weeks,laboratory animals were randomly divided into sham operation con-trol group （Sham）,sham operation plus exercise group （Sham ＋E）,heart failure control group （HF）and heart failure plus exercise group （HF ＋E）.Rats in Sham ＋E group and HF ＋E group performed aerobic treadmill exercise of 10 weeks while those of Sham group and HF group main-tained resting state.Before and after the experiment,cardiac structure parameters including left ven-tricular internal diameter at diastole （LVIDd ）, left ventricular internal diameter at systole （LVIDs）,left ventricular anterior wall diameter at diastole （LVAWDd）,left ventricular anterior wall diameter at systole （LVAWDs）,left ventricular posterior wall diameter at diastole （LVPWDd） and left ventricular posterior wall diameter at systole （LVPWDs）,cardiac function parameters inclu-ding fractional shortening （FS）,left ventricular ejection fraction （LVEF）and heart rate （HR） were detected by ultrechocardiograph;exercise tolerance parameters including exhaust time,exhaust distance and maximal speed were tested by incremental treadmill test.After test,heart tissue was collected for weighting body weight （BW）,left ventricular weight （LVW）,right ventricular weight （RVW）and left ventricular mass index （LVMI）,histopathology study and collagen volume fraction （CVF）by Masson staining.Results：1 ）Compared with Sham group,exhaust time,exhaust dis-tance,maximal speed,BW,LVIDd,FS and LVEF reduced （P 〈0.01 ）while LVW,LVMI, LVAWDd,LVAWDs,LVPWDd,LVPWDs and CVF raised （P 〈0.01）in HF group;2）Compared with HF group,exhaust time,exhaust distance,maximal speed,LVW,LVMI,LVIDd,FS and LVEF increased （P 〈0.01）while HR and CVF decreased （P 〈0.01）in HF ＋E group.Conclu-sion：1）HF resulted in cardiac structure and function remodeling and reduced exercise capacity;2） Aerobic treadmill training of 10 weeks reversed cardiac remodeling of heart failure rats,thus enhan-cing exercise tolerance.
出处
《山东体育学院学报》
北大核心
2015年第1期80-84,共5页
Journal of Shandong Sport University
基金
山东省自然科学基金资助(长期有氧运动对慢性心力衰竭大鼠心脏交感神经功能的调节
立项编号ZR2012HM074)
关键词
有氧训练
心脏
心力衰竭
重塑
运动耐力
aerobic exercise
heart
heart failure
remodeling
exercise tolerance
