摘要
目的探讨血清学定型为Rh D变异型DⅥⅢ型的分子基础。方法对2例Rh D定型弱阳性、阴性反应的标本进行ABD(DⅥ-)血型卡检测、试管法检测Ig M抗-D、Liss/Coombs卡检测Ig G抗-D、直接抗人球蛋白试验、Rh分型卡检测Rh抗原分型、同时采用PCR-SSP进行Rh D基因检测。结果 2例标本Ig M抗-D均阴性,3批Ig G抗-D均4+,ABD(DⅥ-)血型卡D阴性,Rh分型为Ccee,Rh D基因检测结果为D3-6外显子缺失,确认为DⅥⅢ型,基因结构为Rh D-CE(3-6)-D。结论对于Rh D弱阳性的标本可采用分子生物学的方法做基因定型。作为受血者和供血者在临床输血中和临床抗-D同种免疫的预防和监护中的策略是不同的。
Objective To genotype weak D phenotype and study the molecular background of DⅥtypeⅢ. Methods The Rh D blood type of two blood samples were typed by tube test with Ig M anti-D, Liss/Coombs card with Ig G antiD,ABD（DⅥ-）typing card.DAT and Rh phenotype were tested by gel card.The genotype of both samples were identified by PCR- SSP. Results The reactivity of the two samples were negative（one lot Ig M anti- D）,wesk positive（two lots IgManti-D）, 4＋（three lots Ig G anti-D） and negative（anti-DⅥ-）.Both Rh phenotype were Ccee.The two samples were detected negative in exons 3-6 of Rh D gene. The Rh D of both samples was identified DⅥ type Ⅲ,Rh gene was Rh D-CE（3-6）-D. Conclusion Genotype may be tested for weak D phenotype.The genotype of the two samples was DⅥtypeⅢ. We may still consider D VI phenotype individuals as D positive donors and D negative receiptions in our transfusion practice and in clinical anti D allo immune prophylaxis and monitoring.
出处
《北京医学》
CAS
2015年第4期381-383,共3页
Beijing Medical Journal
