摘要
[目的]观察葡萄籽原花青素(GSPE)对氟化钠(Na F)致雄性小鼠肝脏组织氧化损伤的拮抗作用。[方法]将40只健康性成熟昆明种雄性小鼠随机分为4组:对照组(0 mg/kg)、Na F组(20 mg/kg)、GSPE组(200 mg/kg)及Na F+GSPE组[Na F(20 mg/kg)+GSPE(200 mg/kg)],每组10只动物。灌胃染毒,每天1次,连续染毒5周。颈椎脱臼处死,采集肝脏组织样本,计算肝脏脏器系数,检测肝脏组织中谷丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和超氧化物歧化酶(SOD)活性,谷胱甘肽(GSH)和丙二醛(MDA)含量以及总抗氧化能力(T-AOC)水平。[结果]各组肝脏脏器系数组间比较,差异有统计学意义(P<0.05);Na F组高于对照组(P<0.05),(Na F+GSPE)组低于Na F组(P<0.05)。各组AST、ALT含量组间比较,差异有统计学意义(均P<0.05);Na F组和(Na F+GSPE)组高于对照组(P<0.05),GSPE组和(Na F+GSPE)组低于Na F组(P<0.05)。各组GSH、SOD、MDA和T-AOC含量组间比较,差异有统计学意义(均P<0.05);与对照组相比,Na F组和(Na F+GSPE)组小鼠肝脏中GSH、SOD和T-AOC含量较低(P<0.05),MDA含量较高(P<0.05);与Na F组相比,GSPE组和(Na F+GSPE)组GSH、SOD和T-AOC含量较高(P<0.05),MDA含量较低(P<0.05)。析因分析显示:Na F与GSPE对AST、ALT、SOD活力、MDA含量,T-AOC水平存在拮抗作用。[结论]GSPE对Na F致雄性小鼠肝脏氧化损伤具有拮抗作用。
[Objective] To observe the antagonistic effect of grape seed proanthocyanidin extract（GSPE） on oxidative damage of male mice which were treated with excessive sodium fluoride（Na F）. [Methods] A total of 40 healthy and sexually mature male mice were randomly divided into four groups： control, Na F（20 mg/kg）, GSPE（200 mg/kg）, and Na F（20 mg/kg）＋GSPE（200 mg/kg） groups. The animals were fed with deionized water supplemented with non-Na F, Na F, GSPE, or Na F＋GSPE for consecutive five weeks, and sacrificed by cervical dislocation to collect liver tissue samples. We calculated the liver/body weight index and measured the activities of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, and superoxide dismutase（SOD） and the levels of glutathione（GSH）, malondialdehyde（MDA）, and total anti-oxidant capacity（T-AOC）. [Results] The differences in liver/body weight index were statistically significant among the experiment groups（P 〈0.05）. Compared with the control group, the liver/body weight index of the Na F group was increased; compared with the Na F group, that of the Na F＋GSPE group was decreased. The differences in AST and ALT activities among the experiment groups were statistically significant（all Ps 0.05）. Compared to the control group, the activities of AST and ALT of the Na F group and the Na F＋GSPE group were increased（P 〈0.05）; compared to the Na F group, the GSPE group and the Na F＋GSPE group were decreased（P 〈0.05）. The differences in GSH, SOD, MDA, and T-AOC contents among the experiment groups were also statistically significant（all Ps 0.05）. Compared with the control group, the contents of GSH, SOD, and T-AOC of the Na F group and the Na F＋GSPE group were decreased（P 〈0.05）, but the MDA content was increased（P 〈0.05）; compared with the Na F group, the contents of GSH, SOD, and T-AOC of the GSPE group and the Na F＋GSPE group were increased（P 〈0.05）, but the MDA content was decreased（P 〈0.05）. The results of factorial analysis showed an antagonistic interaction between Na F and GSPE on AST, ALT, SOD activities, MDA content, and T-AOC level. [Conclusion] GSPE could antagonize the oxidative damage induced by excessive Na F in male mice.
出处
《环境与职业医学》
CAS
CSCD
北大核心
2015年第4期311-314,共4页
Journal of Environmental and Occupational Medicine
基金
石河子大学科学技术研究发展计划项目(编号:2012ZRKXYQ25)
关键词
氟化钠
葡萄籽原花青素
氧化损伤
拮抗作用
小鼠
sodium fl uoride grape seed proanthocyanidin extract oxidative damage antagonistic effect mouse
