摘要
目的探讨仙地合剂对哮喘发作豚鼠气道上皮的保护作用。方法参照既往文献复制豚鼠哮喘模型。将25只豚鼠随机分为正常对照组、哮喘模型组、强的松组、仙地合剂组、仙地合剂+强的松组5组,每组5只,分别给予2 m L生理盐水、2 m L生理盐水、0.4 mg/100 g体重强的松、0.9 g生药/100 g体重仙地合剂、0.4 mg/100 g体重强的松+0.9 g生药/100 g体重仙地合剂,每日1次灌胃,共2周。2周后取材,通过电子显微镜观察气道上皮和肺泡上皮细胞的超微结构改变。结果扫描电镜显示,正常豚鼠气管纤毛上皮细胞及表面纤毛形态无变化;造模后气管纤毛上皮排列不规则,表面纤毛短而少,形态发生改变;经药物治疗后,气管纤毛上皮细胞排列整齐,纤毛形态恢复正常,其中尤以强的松与仙地合剂合用者最为明显。透射电镜显示,肺泡上皮细胞有类似改变,豚鼠造模后,肺泡上皮细胞形态结构明显发生改变;哮喘豚鼠经过各组药物治疗后,组织形态较不经药物治疗的哮喘模型组趋于正常。结论仙地合剂具有保护哮喘豚鼠气道上皮细胞的作用,说明肺肾同治、攻补兼施是治疗支气管哮喘急性发作的有效途径之一。
Objective To explore the protection effects of Xiandi mixture on airway epithelial cells of asthma guinea pig. Methods Guinea pig asthma model was established based on previous literature. A total of 25 guinea pigs were randomly divided into a normal group,a model group,a prednisone group,a Xiandi mixture group and a prednisone ＋ Xiandi mixture group,5 guinea pigs in each one. The guinea pigs were respectively treated with 2m L normal saline,2m L normal saline,0. 4 mg /100 g prednisone,0. 9 g /100 g Xiandi mixture and 0. 4 mg /100 g prednisone ＋ 0. 9 g /100 g Xiandi mixture. The treatment was given once a day for totally 2weeks. After 2 weeks, the samples were collected; airway epithelial cells and alveolar epithelial cells were observed by electron microscope. Results The scanning electron microscopy indicated that airway ciliated epithelial cell and cilium form was not changed in normal guinea pig; after model establishment,there was irregular arrangement in epithelial cell and form of cilium was changed; after treatment,there was regular arrangement in epithelial cell and normal form of cilium,especially in prednisone ＋ Xiandi mixture group. Transmission electron microscopy indicated that there was similar change in alveolar epithelial cells. After model establishment,structure of epithelial cells was obviously changed; after treatment, the changed structure of epithelial cells tended to be normal. Conclusion The Xiandi mixture has protective effects on airway epithelial cells of asthma guinea pig,indicating that lungkidney concurrent treatment and tonification and purgation in combination is one of the effective methods of acute bronchial asthma.
出处
《北京中医药》
2015年第3期238-240,共3页
Beijing Journal of Traditional Chinese Medicine
基金
首都医学科技发展基金项目(2005-8 F-Ⅱ-029)
关键词
仙地合剂
哮喘
豚鼠
气道上皮细胞
肺泡上皮
超微结构
Xiandi mixture
asthma
guinea pig
airway epithelial cells
alveolar epithelial cells
ultrastructure
