摘要
采用水培方式,设置150 mmol/L Na Cl和不同质量浓度(0、25、50、100和200 mg/L)ALA溶液进行组合处理栀子幼苗,以蒸馏水处理为对照,测定不同处理下栀子叶片的光合色素、相对含水量,相对电导率、丙二醛、游离脯氨酸、可溶性糖、可溶性蛋白的量以及保护酶系统中的超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性。结果表明:150 mmol/L Na Cl溶液处理下的栀子幼苗叶片中相对含水量、光合色素质量分数、可溶性蛋白质量分数以及保护酶系统SOD、POD、CAT活性显著下降,经过一定质量浓度ALA的处理,各生理指标以不同程度地提高,且ALA在25~50 mg/L处理下数值达到最大;同时在一定质量浓度ALA处理下,降低了电导率、丙二醛质量摩尔浓度,且脯氨酸、可溶性糖质量分数显著增加,提高栀子幼苗渗透调节能力。研究结果显示盐胁迫显著抑制栀子幼苗生长,ALA在25~50 mg/L时能够有效缓解盐胁迫对栀子幼苗造成的生理伤害,提高其抗盐性,而ALA在100~200 mg/L处理则有可能加剧盐胁迫。
By water culture,G. jasminoides seedlings living in the circumstance were cultured with 150 mmol / L NaC l and different concentrations(0,25,50,100 and 200 mg / L) ALA,and we used water as control to study the physiological characteristics of G. jasminoides. We measured several physiological indexes of G. jasminoides seedlings under different treatments including photosynthetic pigment,relative water content,relative electric conductivity,malondialdehyde( MDA),free proline( Pro),soluble sugar,soluble protein,superoxide( SOD),peroxidase( POD) and catalase( CAT). The relative water content,photosynthetic pigment content,soluble protein content,superoxide( SOD),peroxidase( POD) and catalase( CAT) of G. jasminoides seedlings under 150 mmol / L NaC l decreased significantly,but appropriate concentration ALA could improve those physiological indexes,especially their value with 25-50 mg / L ALA reached the maximum,and reduced their relative electric conductivity and malondialdehyde( MDA). In addition,appropriate concentration ALA also increased free proline( Pro) and soluble sugar content,so that improved their osmotic regulation ability. Salt stress significantly inhibited the seedling growth of G. jasminoides seedlings. ALA with concentration of 25-50 mg / L could significantly alleviate the physiological damages to the seedlings of G. jasminoides under salt stress,and promote the salt resistance of the seedlings,while 100-200 mg / L ALA could exacerbate the salt stress.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2015年第4期37-41,共5页
Journal of Northeast Forestry University
基金
国家自然科学基金项目(31300555)
中央级公益性科研院所基本科研业务费专项资金项目(RIF2013-12)
安徽省教育厅自然科学研究项目(KJ2013A207)
抗衰老中草药省级科研机构校级委托专项课题(2013KSLZX02)
安徽省高校省级优秀青年人才基金项目(2012SQRL114)
安徽省高校省级自然科学研究项目(KJ2012Z308)
阜阳师范学院卓越人才培养计划(2012ZYJH03)
关键词
栀子
生理特性
5—氨基乙酰丙酸
盐胁迫
Gardenia jasminoides
Physiological characteristics
5-aminolevulinic acid
Salt stress