摘要
目的:利用一种简便易行的方法改善介孔碳(MCN)的分散性,并以其为载体构建纳米给药系统,探索其生物应用的效果。方法:利用TEM观察聚乙二醇-聚乳酸-羟基乙酸共聚物(PEG-PLGA)包裹前后MCN的大小和形态变化。稳定性检测用于评估PEG-PLGA包裹的MCN纳米粒(MCNP)的分散性。热效应实验评价MCNP的近红外(NIR)光热转化能力。构建PEG-PLGA包裹的载阿霉素(DOX)的介孔碳给药系统(MCNPD),从Zeta电荷、载药量、释放以及细胞摄取实验对其进行表征。结果:溶液静置24h后,原始MCN几乎完全聚集沉降,而MCNP仍均匀分散;NIR照射后,MCNP表现出很好的热效应;构建的给药系统MCNPD具有较高的载药量,并且呈p H-依赖释放和NIR-触发释放,同时能够很好地摄取进入细胞。结论:PEG-PLGA包裹以提高MCN分散性的方法简单可行,成功构建了一种具有良好分散性的MCN递药载体,利于生物应用。
Objective： To improve the dispersity of mesoporous carbon nanoparticles（MCN）using asimple and practical method,and further construct a novel drug delivery system to make MCN a desirable carrier for biological application. Methods： TEM was employed to observe the size and morphology of PEG-PLGA coated MCN nanoparticles（MCNP）.The change of dispersitywas evaluated by stability tests. Photothermal heating effect experiment was utilized to evaluate photothermal conversion performance.MCNP-baseddoxolubicin（DOX）-loaded system（MCNPD） wascharacterized by Zeta potential, drug loading content, in vitro release and cellular uptake experiments. Results： Compared to aggregation of MCN, MCNPexhibited moreuniform dispersityafter standing for 24 h. And also,MCNP showed good photothermal conversion efficiencywith NIR irradiation for 5min. The drug delivery system MCNPD presented excellent drug loading capacity, performed p H-responsive and NIR-triggered drug release mechanism, and showed obvious cellular uptake. Conclusion： The method that PEG-PLGA coating to improve the dispersity of MCN was simple and feasible. Anexcellent dispersive MCN drug delivery nanocarrierpossessing the potential of chemo-photothermal therapywas successfully obtained for biological application.
出处
《黑龙江医药》
CAS
2015年第2期248-251,共4页
Heilongjiang Medicine journal
