摘要
目的：建立同时测定痛安注射液中木兰花碱、原阿片碱、黄连碱、白屈菜碱含量的HPLC方法。方法：采刖GeminiC18色谱柱（4．6mm×250mm，5μm），流动相乙腈（A）．0．02mol·L^-1磷酸二氢钾（B）梯度洗脱，流速1．0mL·min^-1，检测波长230nm（木兰花碱），290nm（原阿片碱、白屈菜碱），360nm（黄连碱），柱温30℃。结果：木兰花碱、原阿片碱、黄连碱、白屈菜碱分别在10．856～108．560（r=0．9998），24．712～247．120（r=0．9996），3．464～34．640（r=0．9996），39．296～392．960mg·L^-1（r=0．9999）线性关系良好，回收率在97．5％-101．8％。结论：建立的方法准确可靠，可以用于测定痛安注射液中木兰花碱、原阿片碱、黄连碱、白屈菜碱的含量。
Objective： To establish an HPLC method for content determination of magnoflorine, protopine, coptisine and chelidonine in Tong＇an injection. Method： Gemini C18 （4.6 mm ×250 mm, 5 ×m） was used with the mobile phase of aeetonitrile-0.02 mol ·L^-1 monopotassium phosphate at a flow rate of 1.0 mL ·min^-1 The detection wavelength was set at 230 nm for magnoflorine, 290 nm for protopine and chelidonine, 360 nm for coptisine, and the column temperature was maintained at 30 ℃. Result： Calibration curves of the magnoflorine, protopine, ehelidonine and coptisine in Tong＇an injection showed good linear relationship： 10. 856- 108. 560 （r=0.999 8）, 24. 712-247. 120 （r =0.999 6）, 3. 464-34. 640 （r =0.999 6）, 39. 296-392. 960 mg·L^-1 （r = 0. 999 9 ）. The average recoveries of the components above were between 97.5%-101.8%. Conclusion： The method was feasible, accurate and stable, which could be used for determination of magnoflorine, protopine, coptisine and chelidonine in Tong＇an injection.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第9期50-53,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家“重大新药创制”科技重大专项(2013ZX09402203)
