摘要
通过测定土壤养分，酶活性，pH和微生物标记性磷脂脂肪酸（PLFAs）含量以及鱼腥草多酚和总黄酮含量，分析土壤养分状况、微生物特性及其与鱼腥草多酚和黄酮的关系，了解鱼腥草的土壤条件，提高栽培鱼腥草的品质。不同样地鱼腥草土壤养分、酶活性、pH和微生物的PLFAs含量以及鱼腥草多酚和总黄酮的含量存在差异；不同样地鱼腥草土壤微生物的PLFAs含量趋势为细菌〉真菌〉放线菌〉线虫，且细菌的PLFAs含量占总微生物PLFAs的37．5％～65．0％；土壤多酚氧化酶活性，有效磷，细菌、放线菌及总微生物PFLAs的含量分别与多酚和总黄酮含量呈显著正相关（P〈0．05），真菌PFLAs的含量分别与多酚和总黄酮含量呈显著负相关（P〈0．05）。实验结果暗示了鱼腥草土壤条件可能主要通过土壤酶活性及微生物对土壤氮、磷的转化，改善了土壤养分的有效性，从而影响鱼腥草多酚及总黄酮的代谢累积。
The relationship of nutrients and microorganisms in soils with polyphenols and total flavonoids of Houttuynia cordata were investigated by measuring nutrients, enzyme activity, pH, concentrations of microbe phospholipid fatty acids （PLFAs） in soils, and determining concentrations of polyphenols and total flavonoids of H. cordata. The research is aimed to understand char- acteristics of the planting soils and improve the quality of cultivated H. cordata. The soils at different sample sites varied greatly in nutrients, enzyme activity, pH, microbic PLFAs and polyphenols and all flavonoids. The content of total PLFAs in sample sites was following： bacteria 〉 fungi 〉 actinomyces 〉 nematode. The content of bacteria PLFAs was 37.5% ~65.0% at differ- ent sample sites. Activities of polyphenol oxidease, concentrations of available P and content of PLFAs of bacteria, actinomyces and total microorganisms in soils were significantly and positively related to the concentrations of polyphenols and total flavonoids of H. cordata, respectively （ P 〈 0.05 ）. The Content of fungi PLFAs in soils was significantly and negatively related to concen- trations of polyphenols and total flavonoids of H. cordata, respectively （P 〈 0.05 ）. This study provides evidence that effective- ness of the soil nutrient, which may be improved due to transformation of soil microorganisms and enzymes to N and P in the soils, was beneficial to adaptation of H. cordata adapted to different soil conditions, and significantly affects metabolic accumula- tion of polyphenols and flavonoids of H. cordata.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第8期1444-1452,共9页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81260641)
贵州师范大学博士科研项目
关键词
鱼腥草
多酚
总黄酮
土壤
养分
微生物
PLFAS
Houttuynia cordata
polyphenol
total flavonoid, soil
nutrient
microorganism
PLFAs
