摘要
目的研究通窍活血汤(TQHXD)对拟血管性痴呆(vascular dementia,VD)大鼠学习记忆的作用及对大鼠海马神经元细胞内钙离子浓度的影响。方法通过改良的双侧颈总动脉结扎法(2-VO)建立拟血管性痴呆大鼠模型,通过Morris水迷宫实验对大鼠的学习记忆能力进行检测;流式细胞仪分析检测海马神经元细胞内钙离子(Ca2+)荧光强度。结果与模型组相比,通窍活血汤高剂量、中剂量、低剂量能显著减少血管性痴呆大鼠逃避潜伏期和找到并爬上平台的总路程(P<0.01);高剂量、中剂量及低剂量能明显增加血管性痴呆大鼠进入所在象限的次数、平台所在象限停留的时间、平台所在象限游动的距离(P<0.05),缩短第一次进入平台所在象限的时间(P<0.05);与模型组相比,通窍活血汤高剂量、中剂量、低剂量能明显降低血管性痴呆大鼠海马神经元细胞内Ca2+的浓度(P<0.01,P<0.05)。结论通窍活血汤能够明显改善血管性痴呆大鼠的学习、记忆能力,作用机制可能与其降低血管性痴呆大鼠海马神经元细胞内钙离子浓度,减少异常升高的细胞内钙离子对神经细胞的损伤作用有关。
OBJECTIVE To study activity of tongqiao huoxne decoction （TQHXD） on memory and learning ability of rats with vascular dementia（VD） and its influence on fluorescence intensity of intracellular calcium concentration of hippocampal neurons. METHODS Vascular dementia model was established by using improved bilateral carotid artery ligation method. The Morris water maze experiment was adopted to evaluate the ethology of VD rats. The fluorescence intensity of hippocampus intracellnlar calcium was detected by flow cytometry. RESULTS Compared with the model group, high, middle and low dose group of TQHXD significantly reduce the time of escape latency and the distance of climbing on the platform （P 〈 0. 01 ） . The entries in the second quadrant, the times in the second quadrant and the distance in the second quadrant in relation to high, middle and low dose groups of TQHXD were increasing significantly （ P 〈 0. 05 ） and the latency to first entry in the second quadrant was shorten （P 〈 0. 05 ）. The fluorescence intensity of intracellular calcium in hippoeampal neurons of the high, middle and low dose groups of TQHXD was lower than that of the model group （P 〈 0.01,P 〈 0. 05 ）. CONCLUSION TQHXD can improve the memory and learning ability of rats with VD significantly. The mechanism may be related to reduce the intracellular calcium concentration in the hippocampus, thereby the damage of nerve cells is reduced.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2015年第8期671-675,共5页
Chinese Pharmaceutical Journal
基金
安徽省年度重点科研计划项目(1301043047)
关键词
通窍活血汤
血管性痴呆
钙离子
学习记忆
tongqiao huoxue decoction
vascular dementia
calcium
learning and memory
