摘要
目的以粗毛栓菌Trametes hirsuta lg-9为出发菌株进行原生质体的UV诱变,以期获得漆酶高产菌株。方法优化粗毛栓菌Trametes hirsuta lg-9原生质体的制备方法,并摸索该菌株紫外诱变条件。结果筛选出2株漆酶高产菌株JM-70S3和JM-70S8,分别是出发菌株Trametes hirsuta lg-9漆酶产量的93.57倍和257.24倍。结论原生质体紫外诱变是粗毛拴菌获得漆酶高产菌株的有效方法。
Objective To obtain the high laccase-producing strains,we used the method of UV-mutagenesis to the Trametes hirsuta lg-9 protoplasts.Methods We optimized the protoplast preparation method of bacteria Trametes hirsuta lg-9,and groped for the strain ultraviolet mutagenesis condition.Results JM-70S3 and JM-70S8 were screened out,which produced laccase 93.57 and 257.24 higher than the original strains.Conclusion The UVmutagenesis is an effective method to obtain the high laccase-producing strains of Trametes hirsuta.
出处
《济宁医学院学报》
2015年第2期106-109,共4页
Journal of Jining Medical University
基金
山东省自然科学基金联合专项(编号:ZR2014CL002)
2014山东省高等学校青年骨干教师国内访问学者项目
济宁医学院青年科研基金资助项目(编号:JYQ2011KM012)
济宁医学院博士科研启动基金(编号:094201)
关键词
漆酶
粗毛拴菌
原生质体
紫外诱变
Laccase
Trametes hirsuta
Protoplast
UV mutagenesis
