摘要
【目的】优化竹荪菌托多糖的提取工艺,为其开发利用提供技术支持。【方法】以竹荪菌托为原料,通过单因素试验分析提取温度、提取时间、液料比、提取次数对竹荪菌托多糖提取率的影响,并在此基础上采用Box-Benhnken响应面法建立以竹荪菌托多糖提取率为响应值的二次回归数学模型。【结果】通过响应面分析建立竹荪菌托多糖提取回归方程为:Y=12.96+0.29A+0.13B+0.17C-0.13AB-0.039AC-0.014BC-0.34A2-0.27B2-0.34C2(A为提取温度,B为提取时间,C为液料比,Y为竹荪菌托多糖提取率,R2=0.9551),该模型拟合度好;并确定竹荪菌托多糖提取的影响因素顺序为:提取温度>液料比>提取时间,其中提取温度和液料比对竹荪菌托多糖提取率有极显著影响(P<0.01),提取时间有显著影响(P<0.05),但双因素交互作用对竹荪菌托多糖提取率无显著影响(P>0.05);竹荪菌托多糖最佳提取工艺条件为:在提取温度82℃、液料比62∶1 m L/g的条件下提取3.1 h、提取1次,竹荪菌托多糖提取率为13.016%,与模型预测理论值13.040%接近。【结论】采用响应面法优化竹荪菌托多糖提取工艺具有可行性,可用于实际生产。
[Objective]The extraction parameters of polysaccharide in volva of Dictyophora indusiata were optimized to provide technical support for its further development. 【Method 】Volva of Dictyophora indusiata was used as material. Single-factor experiments and response surface methodology were used to select appropriate levels on extraction temperature,extraction time,liquid-to-material ratio and extraction times. A quadratic regression model describing the effects of extraction conditions on the extraction rate of polysaccharide was developed by Box-Behnken experimental design. 【Result】The quadratic multinomial regression equation of polysaccharide extraction in volva of Dictyophora indusiata was established as Y=12.96＋0.29A＋0.13B＋0.17C-0.13AB-0.039AC-0.014BC-0.34A^2-0.27B^2-0.34C^2（A ： extraction temperature, B： extraction time, C： liquid-material ratio,Y： extraction rate of polysaccharide,R^2=0.9551）,and the model fitted well with the experimental data. The influencing order of parameters on the extraction rate was extraction temperature liquid-to-material ratio extraction time. Extraction temperature and liquid-to-material ratio extremely affected the extraction rate of polysaccharide and extraction time was less influential. However, interactive function of two factors slightly affected the extraction yield of polysaccharide. The optimal extraction conditions for polysaccharide were determined as follows： extraction temperature at 82 ℃, extraction time of 3.1 h and liquid-to-material ratio of 62∶1 m L/g.After one extraction under the optimized conditions, the extraction rate of polysaccharide reached 13.016%,basically close to the theoretical content of 13.040%. 【Conclusion】The response surface methodology is a workable method to optimize the extraction parameters of polysaccharide in volva of Dictyophora indusiata,so it could be used in practical production.
出处
《南方农业学报》
CAS
CSCD
北大核心
2015年第3期480-485,共6页
Journal of Southern Agriculture
基金
国家自然科学基金项目(81160336)
贵州省科技厅农业攻关计划项目(黔科合NY字[2011]3055号)
贵州省教育厅基金项目(黔省专合字[2010]39号)
贵州省科学技术基金项目(黔科合J字[2010]2188)
关键词
竹荪菌托
多糖
响应面法
工艺优化
volva of Dictyophora indusiata
polysaccharide
response surface methodology
technique optimization
