摘要
为探究叶用莴苣2个Hsp70基因Ls Hsp70-2711和Ls Hsp70-3711与叶用莴苣的抽薹是否相关,采用实时荧光定量PCR技术(RT-PCR),以2个品种的叶片为试材,分析这2个基因在耐热型品种G-S59和热敏型品种P-S11 2个叶用莴苣品种抽薹前后的相对表达量。研究结果表明:首先,这2个Hsp70基因在抽薹前后的表达量表现出显著差异,所以推测与叶用莴苣的抽薹性具有一定关联;其次,Ls Hsp70-2711基因在2个叶用莴苣品种中的表达趋势均为抽薹前显著高于抽薹后,且在相同处理下G-S59中的表达量又明显高于P-S11中的表达量,Ls Hsp70-3711基因与Ls Hsp70-2711基因在2个品种中的表达情况相反。叶用莴苣热激蛋白基因Ls Hsp70-2711和Ls Hsp70-3711除与叶用莴苣的耐热性相关外,还与其抽薹性具有一定相关性。
Based on a heat-resistant type G-S59 and a heat-sensitive type P-S11, in this study, we analyzedthe relationship between bolting and two heat shock protein genes in Lactuca sativa L.（Ls Hsp70-2711 geneand Ls Hsp70-3711 gene） with the help of real-time quantitative PCR（q RT-PCR）. The result showed that theexpression of the two Hsp70 genes in lettuce varied significantly before and after bolting, based on which wecould conjecture that there was a relationship between bolting and the two Hsp70 genes in lettuce. In addition,in either type, the relative expression of Ls Hsp70-2711 after bolting was evidently higher than that beforehand.Moreover, when under the same treatment, the expression of Ls Hsp70-2711 gene in heat-resistant type G-S59 was much higher than that in heat-sensitive type P-S11 while the expression of Ls Hsp70-3711 gene had theopposite result. Therefore we deduced that the two Hsp70 genes not only had correlation with heat-resistancein lettuce, but also had some extent relationships with bolting.
出处
《中国农学通报》
2015年第10期58-62,共5页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金项目"叶用莴苣高温抽薹相关蛋白的鉴定及其互作分析"(31372057)
国家自然科学基金项目"叶用莴苣高温胁迫响应因子Ls Hsp70的功能研究"(31401883)
现代农业产业技术体系建设专项"北京市叶类蔬菜创新团队"(blvt-02)
北京农学院促进人才培养综合改革专项计划"叶用莴苣高温胁迫中Ls Hsp70家族基因的表达模式研究"(BNRC&GG201411)
北京市农业科技项目"生菜耐热新品种繁育及安全生菜技术实验示范项目资助"(20140134)
科研基地建设-科技创新平台"蔬菜创新平台建设"(PXM2014_014207_000028)
