摘要
目的：研究枸骨叶、茎枝、果实萃取物的抗菌与抗氧化活性。方法：采用纸片扩散法,分别测定枸骨叶、茎枝、果实的石油醚、氯仿、乙酸乙酯、正丁醇、水萃取物对金黄色葡萄球菌、枯草芽孢杆菌、大肠埃希菌、伤寒杆菌的抗菌活性;选取对3种菌的抑菌圈直径均不小于10 mm且相对较大的萃取物,采用倍比稀释法测量其最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。采用分光光度法测定过氧化值（POV）,比较猪油（空白对照）和不同溶剂萃取物加样16 d内的抗氧化作用。结果：除石油醚萃取物无抗菌活性、所有萃取物对伤寒杆菌无抗菌活性外,叶、茎枝、果实的抑菌圈直径分别为8~26、7~21、7~21 mm。针对金黄色葡萄球菌、枯草芽孢杆菌、大肠埃希菌,枸骨叶氯仿萃取物的MIC、MBC分别为0.125、0.5、0.5 mg/ml,0.25、1.0、0.5 mg/ml;枸骨叶乙酸乙酯萃取物的MIC、MBC分别为0.062 5、0.25、0.25 mg/ml,0.062 5、0.5、0.5 mg/ml。枸骨不同溶剂萃取物的POV曲线均在空白对照曲线的下方。结论：枸骨叶乙酸乙酯萃取物对金黄色葡萄球菌的抗菌活性最强,枸骨叶、茎枝、果实的萃取物均有抗氧化作用。
OBJECTIVE： To determine the antibacterial and antioxidant activities of the leaves, stems and branches and fruits of llex cornuta. METHODS： Disk diffusion method was used to detect the antibacterial activities of petroleum ether, chloroform, ethyl acetate, N-butanol and water soluble extracts from the leaves, stems and branches and fruits of L cornuta against Staphylococ- cus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi. The strains with a bacteriostatic ring no less than 10 mm in di- ameter and relatively large were selected, for which doubling dilution method was used to determine the minimal inhibitory concen- trations （MIC） and minimal bactericidal concentrations （MBC）. Spectrophotometry was used to determine the peroxide value （POV） to compare the antioxidation between the lard （blank control） and extracts by different solvents within 16 d. RESULTS： Except the petroleum ether extract, all extracts had an antibacterial activity, but none of them had the antibacterial activity against Sal- monella typhi. The bacteriostatic rings of the extracts from the leaves, stems, branches and fruits were 8-26, 7-21 and 7-21 mm re- spectively. The MIC and MBC of the chloroform extract from the leaves of L cornuta against Staphylococcus aureus, Bacillus subti- lis and Escherichia coli were 0.125, 0.5, 0.5 mg/ml, and 0.25, 1.0, 0.5 mg/ml, respectively. The MIC and MBC of the ethyl ace- tate extract from the leaves of I. cornuta were 0.062 5, 0.25, 0.25 mg/ml, and 0.062 5, 0.5, 0.5 mg/ml, respectively. The POV curves of all extracts from 1. cornuta by different solvents were all below those of the blank control. CONCLUSIONS ： The ethyl acetate extract from the leaves of 1. cornuta has the highest antibacterial activity against Staphylococcus aureus, and all extracts of leaves, stems, branches and fruits of I. cornuta have antioxidation.
出处
《中国药房》
CAS
北大核心
2015年第13期1776-1778,共3页
China Pharmacy
基金
日照市应用技术研究与开发计划项目(No.2013JHSZ017)
济宁医学院重点科研项目(No.JY2013KJ011)
关键词
枸骨
抗菌活性
抗氧化活性
Ilex cornuta
Antibacterial activity
Antioxidant activity
