摘要
该研究通过观察通心络胶囊对自发性2型糖尿病KK/Upj-Ay小鼠坐骨神经细胞凋亡的影响,试探讨其改善糖尿病周围神经病变(DPN)的机制。实验以KK/Upj-Ay小鼠为DPN模型,随机分为模型组、通心络低、中、高剂量组(1,2,4 g·kg^(-1)),另设C57BL/6小鼠为对照组。灌胃给药12周,测定热痛觉阈值、运动神经传导速度(MNCV)和感觉神经传导速度(SNCV);流式细胞术测定细胞凋亡率;Real-time PCR和Western blot法测定坐骨神经B细胞白血病/淋巴瘤相关抗原2(Bcl-2)、白血病/淋巴瘤相关抗原相关X蛋白(Bax)、半胱氨酸天冬氨酸蛋白酶3(Caspase-3)mRNA和蛋白的表达;Western blot法测定坐骨神经p38MAPK,p-p38MAPK蛋白水平。研究发现通心络胶囊能不同程度的升高痛觉阈值,增加MNCV和SNCV;通心络胶囊能降低坐骨神经细胞凋亡率;降低Bax和Caspase-3的表达,升高Bcl-2表达;通心络胶囊能降低p-p38MAPK蛋白水平。结果表明通心络胶囊能够通过抑制p38MAPK磷酸化抑制DPN小鼠细胞凋亡,从而发挥糖尿病周围神经的保护作用。
To investigate the effects of Tongxinluo capsule on sciatic nerve apoptosis in spontaneous type Ⅱ diabetic KK/Upj-Ay mice, in order to explore its mechanism for improving diabetic peripheral neuropathy （DPN）. KK/Upj-Ay mice were selected as the DPN animal model and randomly divided into the model, Tongxinluo low, middle and high group （1, 2, 4 g·kg^-1）. C57BL/6 mice were selected as the control group. Mice were given intragastrically for 12 weeks. Paw withdrawal latency, motor nerve conduction velocity （MNCV） and sensory nerve conduction velocity （SNCV） were detected. Apoptotic rate were detected by FCM. Bcl-2, Bax, Caspase-3 mRNA and protein expression in sciatic nerve were examined by Real-time PCR and Western blot. p38MAPK, p-p38MAPK expression were examined by Western blot. In this study,the authors found that Tongxinluo capsule could increase paw withdrawal latency, MNCV and SNCV. Apoptotic rate of sciatic, the expression of Bax and caspase-3 were lower, while Bcl-2 expression was higher in Tongxinluo group than those in model mice. The expression of p-p38MAPK significantly decreased in Tongxinluo group. The results showed that Tongxinluo capsule has protective effects on diabetic peripheral neuropathy of mice via inhibiting cell apoptosis and suppressing the expression of p-p38MAPK.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2015年第7期1396-1399,共4页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2011ZX09101-004-02)
