摘要
目的：建立测定连翘不同部位中松脂醇β－D葡萄糖苷、表松脂醇β－D葡萄糖苷、连翘苷、连翘脂素和连翘酯苷5种成分的高效液相荧光定量方法（ HPLC －FLD ）。方法：采用色谱柱 Dima C18（4.6mm ×250mm，5μm），流动相甲醇（A）－水（B）梯度洗脱系统，流速1．0mL／min。木脂素类成分荧光检测激发波长为270nm，发射波长为340nm；咖啡酸苯乙醇苷类成分激发波长为320nm，发射波长为460 nm。结果：5种成分均达到基线分离，各成分均有较宽的线性范围和良好的线性关系（ r＞0．998）；回收率在95．68％～102．59％。结论：连翘不同部位中5种成分的定性定量测定方法准确、灵敏、实用，为连翘的质量评价提供理论依据。
Objective：To establish an high performance liquid chromatography and fluorescence detection （ HPLC-FLD） method for simultaneous determination of five components such as （＋）-pinoresinol-β-D-glucoside, （＋）-epipi-noresinol-β-D-glucoside, phillyrin, phillygenin and forsythoside in different parts of Fosrythia suspensa.Methods：The separation was carried out in a reversed phase C 18 column （4.6mm ×250mm ,5μm） by a gradient elution program with a mobile phase gradient consisting of methanol and water at the flow rate of 1.0 mL/min.The lignan profile was re-corded at λex/λem =270/340nm, while the caffeoyl phenylethanoid glycosides profile was recorded at λex/λem =320/460nm.Results：The five components were separated to baseline.Each component had a wide linear range and a good linear relationship （r〉0.998）;recoveries were from 95.68%to 102.59%.Conclusion：The qualitative and quantita-tive analysis of F.suspensa samples by using HPLC-FLD method is accurate, sensitive and practical ,which provides a scientific basis for the quality evaluation of F.suspensa.
出处
《中医药学报》
CAS
2015年第2期18-22,共5页
Acta Chinese Medicine and Pharmacology
基金
国家科技部国家重大新药创制专项(2012ZX09304005004)
黑龙江省博士后启动基金(LBH-Q13158)
黑龙江省博士后特别资助项目(LBH-Z10019)
黑龙江中医药大学校基金(035094)
