摘要
目的:探讨CD4+CD25+调节性T细胞、CD4+CD25+Foxp3+调节性T细胞及其他免疫细胞在川崎病(KD)免疫学发病机制中的作用。方法:选取急性期KD患儿58例,其中31例无冠状动脉损害(CAL),27例有CAL,另选取同期健康儿童30例作为对照组。采用流式细胞仪测定外周血CD4+CD25+调节性T细胞、CD4+CD25+Foxp3+调节性T细胞及其他免疫细胞比例并分析其变化规律。结果:急性期KD患儿外周血中,CD4+CD25+、CD4+CD25+Foxp3+、CD3+CD8+、CD3-CD(16+56+)细胞比例明显降低,CD4+/CD8+、CD19+CD23+、CD3-CD19+细胞亚群比例明显增高(P均<0.01)。无CAL的KD患儿与有CAL的KD患儿调节性T细胞亚群比较差异无统计学意义(P>0.05)。结论:KD急性期存在免疫调节功能紊乱,可能与CD4+CD25+调节性T细胞和CD4+CD25+Foxp3+调节性T细胞减少密切相关。
Objective： To investigate the role of CIM＋CD25＋ regulatory T cells, CD4＋ CD25＊ Foxp3＋ regulatory T cells and the other immune cells subsets in immunological pathogenesis of Kawasaki disease （KD）. Methods： Total 58 children with KD were enrolled in KD group, including 27 cases with coronary artery lesion （CAL） and 31 cases without coronary artery lesion （non-CAL）, 30 agematched health children were included in control group. Applying flow cytometer （FCM） to determine the proportion of CD^4＋ CD25＋ regulatory T ceils, CD4＋ CD25 ＋ Foxp3 ＋ regulatory T cells and the other immune cells subsets. Results： The proportion of CD4＋ CD25 ＋ regulatory T cells and CD^4＋CD25＋ Foxp3＋, CD3＋CD8＋, CD3-CD（ 16＋56＋） cells in peripheral blood from children with KD at acute phase were significantly lower than those of control group （ P〈0.01 ）. The proportion of CD4＋/CD8＋, CD19＋ CD23＋, CD3- CD19＋ increased significantly. There was no statistical difference between the proportion of immune cells subsets from cases with CAL and cases with non-CAL. Conclusion： Immune dysfunction is involved in the acute stage of KD, the decrease of CD4＋ CD25＋ and CD4＋ CD25＋ Foxp3＋ regulatory T cells may be correlated with this.
出处
《儿科药学杂志》
CAS
2015年第5期7-9,共3页
Journal of Pediatric Pharmacy
