摘要
目的筛选利胆排毒口服液抗内毒素活性部位。方法采用鲎试剂凝集试验法,对利胆排毒口服液不同极性部位抗内毒素作用进行了体外观察。结果各部位均呈现一定的内毒素破坏作用,而水溶性部位在0.01g/m L仍然对内毒素有破坏作用。结论水溶性部位是抗内毒素主要活性部位,乙酸乙酯和正丁醇部位次之,石油醚部位在处方量浓度下无抗内毒素活性。
Abstracts：Objective To screen out the active fractions with anti -endotoxin activity from gallbladder -benefiting and toxin -expelling oral liquid.Methods Using limμLus amebocyte lysate agglutination testmethod, to observe the anti -endotoxin activity of different chemical fractions from gallbladder -benefiting and toxin -expelling oral liquid in vitro.Results Every chemical fraction had some anti -endotoxin effect,water fraction still antagonized the endotoxin in 0.01g/mL.Conclusion Water fraction was the main active fraction of anti -endotoxi-nand then the ethyl acetate and n -butanol extracts,petroleum ether fraction had no anti -endotoxin activity in the prescription volume con-centration.
出处
《湖北中医药大学学报》
2015年第2期48-50,共3页
Journal of Hubei University of Chinese Medicine
基金
湖北省教育厅中青年人才项目(Q20121610)
关键词
利胆排毒口服液
抗内毒素
极性部位
鲎试剂凝集试验法
gallbladder -benefiting and toxin -expelling oral liquid
anti -endotoxin
chemicalfraction
limulus amebocyte lysate agglu-tination testmethod
