摘要
目的：建立补肾壮骨膏中补骨脂素的含量测定方法。方法：采用高效液相色谱法，Prontosil 120-5-C18色谱柱（250 mm×4.6 mm，5μm），流动相为甲醇-0.2%磷酸溶液（45∶55）；流速1.0 mL/min；检测波长246 nm；柱温30℃；进样量10μL。结果：补骨脂素对照品进样量在0.00994～0.49670μg范围内，进样量与峰面积呈良好的线性关系（r=0.9999），补骨脂素平均加样回收率为98.20%，RSD为1.58%（n=6）。结论：本方法操作简便，准确可靠，专属性强，重现性好。适宜用于测定补肾壮骨膏中补骨脂素的含量。
Objective：To establish a HPLC method for the content determination of psoralen in bushen zhuanggu plaster.Methods：HPLC was carried out on Prontosil 120-5-C18 column（250 mm×4.6 mm,5μm） with a mobile phase of methanol-0.2% phosphoric acid（45∶55）, the flow rate was 1.0 mL/min at UV detection wavelength of 246 nm,the column temperature was at 30 ℃,and the sample size was 10μL.Results：A good linear relationship of the reference material of psoralen was obtained within the range of 0.009 94 ~0.496 70μg,r= 0.999 9. The average recovery of psoralen is 98.20%,RSD = 1.58%（n= 6）.Conclusion：The method is convenient,accurate and specific with a good reproducibility, it is suitable for the content determination of psoralen in bushen zhuanggu plaster.
出处
《中国执业药师》
CAS
2015年第4期20-23,共4页
China Licensed Pharmacist
