摘要
目的：建立HPLC法测定不同厂家牛黄解毒片中黄芩苷的含量。方法采用HPLC法检测牛黄解毒片中黄芩苷的含量，色谱条件：色谱柱：Diamonsil C18柱（250 nm×4.6 mm，5μm）；流动相：甲醇-水-磷酸（50：50：0.2），流速：0.8 ml/min；检测波长：280 nm；柱温：20℃。进样量：20μl。结果黄芩苷在浓度为0.02-0.16 mg/ml内峰面积呈良好的线性关系（r=0.9990），回收率分别为92.04%、94.10%和96.24%，RSD≤2.27%（n=3）；不同厂家牛黄解毒片中黄芩苷的含量从0.98%到2.49%不等。结论 HPLC法操作简便、灵敏，具有良好的重复性和稳定性，可用于牛黄解毒片中黄芩苷的质量控制。
Objective：To establish a high performance liquid chromatographic（ HPLC）method for the determination of baicalin in Niuhuang Jiedu Tablets from different drug manufacturers. Methods：The HPLC separation was performed on a Diamonsil C18 column（5m,250nm 4. 6mm）,with a mobile phase as Methanol-water-phosphoric acid（50： 50： 0. 2）at a flow rate of 0. 8mL/min. The detection wavelength was 280 nm and the column temperature was 20℃. The injection vol-ume was 20μl. Resluts：The method showed a good linearity of the Baicalin in the range of 0. 02 -0. 16mg/ml（ r =0. 9990）. The recovery was 92. 04%,94. 10% and 96. 24%,RSD≤2. 27%（n=3）. The baicalin in Niuhuang Jiedu Tablets from different drug manufacturers were different from 0. 98% to 2. 49%. Conclusion：This method is simple and rapid with repeatability and stability. It is suitable for the quality contorl of baicalin in Niuhuang Jiedu Tablets.
出处
《泰山医学院学报》
CAS
2015年第1期61-63,共3页
Journal of Taishan Medical College
