摘要
目的观察截断逆挽方对慢加急性肝衰竭(ACLF)模型大鼠肝细胞超微结构,周期蛋白Cyclin E及转录因子DP-1表达的影响,并考察模型24 h病死率和生存时间,从提高疗效、降低死亡、改善肝细胞超微结构损伤和调控肝细胞代偿性增殖的角度,分析该方干预ACLF的部分疗效和作用机制。方法 Wistar大鼠61只,随机分为正常组、模型组及截断逆挽方组。采用猪血清免疫诱导大鼠肝纤维化或肝硬化模型,再给予D-Gal N/LPS一次性联合腹腔注射进行急性攻击,建立慢加急性肝衰竭大鼠模型。截断逆挽方组在急性攻击后给予水煎液灌胃连续3 d,模型组和截断逆挽方组大鼠分别在4、8、12 h处死,取肝组织,进行电镜超微结构观察,Elisa法测定Cyclin E及其转录因子DP1的表达,运用IPP 6.0软件进行图像分析,自动计算出阳性物质的IOD值。结果截断逆挽方可以延长模型的存活时间,与模型组比较,差异有统计学意义(P<0.05),在一定程度上减轻ACLF大鼠肝细胞超微结构的损伤;截断逆挽方组Cyclin E、DP1 IOD值在8 h时比4 h低,12 h比8 h有增加(P<0.01),而模型组Cyclin E、DP1 IOD则呈持续下降(P<0.05)。结论截断逆挽方可以延长模型大鼠的存活时间,并能改善肝细胞超微结构,对肝细胞代偿性增殖有一定的调控作用。
Objective To observe the effects of truncation and inversion prescription on the ultrastructure,Cyclin E and its transcription factor DP-1 in liver cells of model rats with acute-on-chronic liver failure （ACLF）, to explore 24-hour mortality and survival time of rat models and to analyze the therapeutic effects and action mechanism of the prescription on ACLF.Me thods Sixty -one Wistar rats were randomly divided into three groups： normal control group, model group and Chinese traditional medicine group （TCM group）.The rat models with hepatic fibrosis or hepatic cirrhosis were established by immune induction of pig serum,then D-GalN/LPS one-time combination intraperitoneal injection was performed to establish the rat models with acute hepatic failure.After the animal models were established,the rats were treated by truncation and inversion prescription decoction through gavage for 3 days,then the rats in model group and TCM group were sacrificed respectively at 4h,8h,12h,and the ultrastructure changes of liver tissues were observed by electron microscopy, and the expression levels of Cyclin E and its transcription factors DP-1 were detected by ELISA.The images were analyzed by IPP6.0 software, and IOD value in positive substance was automatically calculated.Results The survival time in TCM group was significantly longer than that in model group （ P 〈0.05）, furthermore,the ultrastructural injury of liver cells in TCM group was relieved at some extent; the IOD vlue and levels of Cyclin E ,DP-1 in TCM group at 8h were obviously lower than those at 4h,however,which in 12h were significantly increased,as compared with those at 8h （ P 〈0.01）,but IOD value and levels of Cyclin E, DP-1 in model group were decreased continually.Conclusion Truncation and inversion prescription can prolong survival time of model rats, and can improve the ultrastructure of liver cells, which has regulative effects on compensatory proliferation of liver cells at some extent.
出处
《河北医药》
CAS
2015年第9期1285-1289,共5页
Hebei Medical Journal
基金
北京市自然科学基金资助项目(编号:7112017)
关键词
截断逆挽方
肝衰竭
慢加急性Cyclin
E
DP-1
truncation and inversion prescription
acute-on-chronic liver failure
ultrastructure
Cyclin E
DP-1
