摘要
目的:建立桑白皮中1种原型入血成分的含量测定方法。方法:以血清药物化学方法追踪确定桑白皮原型入血成分;采用Dikma Diamonsil C18色谱柱(4.6 mm×250 mm,5μm),柱温30℃,甲醇-0.1%甲酸水为流动相,流速1.0 m L·min-1,检测波长326 nm,测定桑白皮药材中原型入血成分的含量。结果:在大鼠含药血浆中检测到7个移行成分,鉴定入血原型成分桑皮苷A;此成分线性范围为0.1-1.0μg(r=0.999 9);平均回收率为98.2%(RSD=1.38%,n=6)。结论:建立的含量测定方法简便可行重复性良好,测定14批不同来源的桑白皮中桑皮苷A含量差异较大,可将此成分作为桑白皮质量控制指标成分。
This study was aimed to establish a method for determination of composition migrating to blood from Morus alba L. The sample of prototype composition migrating to blood from M. alba was identified by serum pharmacochemistry. The separation was made on a Dikma Diamonsil C18 column （4.6 mm × 250 mm, 5 μm）. The column temperature was 30℃. The mobile phase consisted of methanol-0.1% phosphoric acid solution. The flow rate was 1 mL·min^-1. And the detection wavelength was 326 nm. The content of composition migrating to blood from M. alba was determined. The results showed that 7 migrating components were detected. The calibration curve of mulberroside A migrating to blood was linear in the range of 0.1-1.0 ℃g （r=0.999 9）. The average recovery rate was 98.2% （RSD=1.38%, n=6）. It was concluded that the method was simple, practical and reproducible. There was big difference in the content determination of mulberroside A from 14 batches of M. alba from different sources. This method can be used in the quality control of M. alba.
出处
《世界科学技术-中医药现代化》
2015年第1期134-137,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
四川省教育厅重点项目(2010A036):建立血清化学成分HPLC指纹图谱测定中药半衰期的方法学研究
负责人:张旭
关键词
桑白皮
入血成分
桑皮苷A
含量测定
Morus alba L., composition migrating to blood, mulberroside A, content determination
