摘要
利用超声雾化-固相萃取法萃取,气相色谱法和气相色谱-质谱法测定了小茴香中的挥发油.采用正十四烷作为洗脱溶剂,体积为50μL、吹扫气流速为200 m L/min,吹扫时间为5 min.利用优化后的方法测定了小茴香中挥发性成分的种类和含量,与水蒸馏提取法所得结果相似.测定样品溶液中挥发性成分的回收率为65.8%~102.0%,相对标准偏差小于8.0%.
The ultrasonic nebulization extraction coupled with solid-phase extraction （UNE-SPE） followed by gas chromatography and gas chromatography-mass spectrometry was developed and applied to extract and determine the vol- atile compounds from foeniculum vulgare mill. Experimental parameters, including the kind of extraction solvent （ntetradeeane） , solvent volume （50 μL） , flow rate of purging gas （200 mL/min） and purging time （5 min） , were ex- amined and optimized. The contents of the constituents from foeniculum vulgare mill obtained by the proposed method were closed to those obtained by hydro-distillation （HD）. The recoveries for determining the four constituents are be- tween 65.8% - 102.0% and RSDs are less than 8.0% .
出处
《信阳师范学院学报（自然科学版）》
CAS
北大核心
2015年第2期231-234,共4页
Journal of Xinyang Normal University(Natural Science Edition)
基金
国家自然科学基金项目(20801022)
吉林大学自然科学研究项目(421031471412)
关键词
超声雾化
固相萃取法
小茴香
挥发性成分
气相色谱-质谱
ultrasonic nebulization extraction
solid-phase extraction
foeniculum vulgare mill
volatile com-pounds
gas chromatography-mass spectrometry
