摘要
目的：建立温阳通痹丸的质量标准。方法：采用薄层色谱法（TLC）鉴别温阳通痹丸中的赤芍和麻黄,采用高效液相色谱法（HPLC）测定赤芍中芍药苷的含量。色谱条件：色谱柱为岛津SHIADZU VP-ODS（250 mm×4.6 mm,5μm）,流动相为乙腈-水（18∶82）,流速1.0 m L/min,柱温30℃,检测波长为230 nm。结果：TLC的斑点清晰,阴性对照无干扰;芍药苷在10.22-204.40μg/m L（r=0.999 9）范围内线性关系良好,平均回收率为98.45%,RSD为0.7%（n=6）。结论：所建标准可用于温阳通痹丸的质量控制。
Objective：To establish the quality standard of wenyang tongbi pills. Methods：TLC was used to determine Paeonide radix rubra and Ephedrae herba in wenyang tongbi pills,and HPLC was used to determine the content of paeoniflorin. A chromatographic column of SHIADZU VP-ODS（250 mm × 4.6 mm,5 μm）was used with a mobile phase of acetonitrile-water（18 ∶82）at a flow rate of 1.0 m L/min, the detection wavelength was at230 nm and the room temperature was at 30 ℃. Results：The spots of TLC were fairly clear and the negative control without interference. A good linear relationship of paeoniflorin was from 10. 22 to 204. 40 μg/m L（ r =0.999 9）. The average recovery was 98.45%, RSD was 0.7%（n = 6）. Conclusion：The established standard is applicable for the quality control of wenyang tongbi pills.
出处
《中国执业药师》
CAS
2015年第5期28-31,共4页
China Licensed Pharmacist
