摘要
目的通过分析慢性HBV感染者不同免疫状态外周血T、B细胞亚群的占淋巴细胞的百分比探讨其相互关系。方法选取2010年2月至2013年3月50例在该院就诊医治的慢性HBV感染者作为试验组,按照免疫状态的不同将试验组分为21例免疫耐受组和29例免疫清除组,同时选取20例健康成人作为正常对照组,采用流式细胞术检测外周血T、B细胞亚群的百分比,对3组的临床资料进行了对比。结果与对照组相比,免疫耐受组和免疫清除组患者的B细胞、CD8+T细胞的百分比明显增高;而免疫耐受组和免疫清除组患者CD3+T、CD4+T细胞的百分比和CD4+/CD8+的比值均明显降低,不同组间相比较差异有统计学意义(P<0.05)。结论外周血T、B细胞亚群的百分比可作为慢性HBV感染者免疫状态的参考指标,常规检测该项目对了解HBV感染者的不同免疫状态以及疾病的治疗和疫苗接种等具有重要的指导作用,值得临床推广应用。
Objective To analyze the different immune states and peripheral blood T and B lymphocyte subsets in the patients with chronic HBV infection for investigating their correlation. Methods 50 patients with chronic HBV infection in our hospital from February 2010 to March 2013 were selected as the experiment group and divided into two groups according to the different im- mune states,immune tolerance group（21 cases） and immune clearance group （29 cases）. Contemporaneous 20 healthy adults were selected as the control group. The percentage of peripheral blood T, B cell subsets was detected by using the flow cytometry. The clinical data were compared among three groups. Results The percentages of B cells and CD8＋ T cells in the immune tolerance group and immune clearance group were significantly increased compared with the control group ~ while the percentages of CD3＋ T and CD4＋T cells,CD4＋/CD8＋ ratio in the immune tole^ance group and immune clearance group were significantly decreased, differences between the groups were statistically significant（P〈（0.05）. Conclusion The percentage of peripheral blood T and B cell subsets could serve as the reference indicator of the immune state in the patients with chronic HBV infection. The routine detection of this item has an important guidance role for understanding the different immune states, treatment and vaccination in the patients with HBV infection and is worthy of being clinically promoted.
出处
《国际检验医学杂志》
CAS
2015年第9期1248-1249,共2页
International Journal of Laboratory Medicine
