摘要
斑马鱼TATA结合蛋白(TBP)是转录过程中的重要起始因子。利用生物信息学方法对斑马鱼TBP的理化性质、物种间同源性、保守结构域、跨膜区、亲水性/疏水性、蛋白质二级结构、蛋白质三级结构、蛋白质相互作用进行预测分析。分析表明,斑马鱼TBP全长302个氨基酸,等电点9.8,属于TATA结合蛋白超家族,不含跨膜区,属于亲水蛋白;二级结构以无规则卷曲为主,含5个α螺旋区和8个β折叠区,三维建模空间结构可信度98.9%,进一步分析建模结果可靠;与斑马鱼TBP相互作用的蛋白质均为转录因子或TFⅡD复合物组分。分析结果对于深入研究斑马鱼TBP在基因转录中的作用具有一定的理论指导意义。
TATA-binding protein （TBP） is important in the process of transcription initiation in zebrafish. By means of bioinformatic methods, TBP of zebrafish is illustrated and analyzed by physicochemical proper- ty, sequence homology among different species, conserved domains, transmembrane structures, hydrophilici- ty/hydrophobicity, protein secondary structure, protein tertiary structure, as well as protein-protein interac- tions. The results showed that zebrafish TBP consists of 302 amino acids, with isoelectric point of 9.8. This pro- tein belongs to the TBP superfamily without transmembrane structure, and it is a hydrophilic protein. The sec- ondary structure analysis showed that it contained 5 a helices and 8β sheets and random coil was the major structure element. The reliability of predicted three-dimensional structure of zebrafish TBP was up to 98.9%. Further analysis proved that the predicted protein structure was stable. It showed that the 10 most relevant in- teraction proteins with the zebrafish TBP were all transcription factors or TF ⅡD complex members. Therefore, the results provided great information about zebrafish TBP in transcription regulation for further research.
出处
《生命科学研究》
CAS
CSCD
2015年第2期119-123,136,共6页
Life Science Research
基金
国家自然科学基金资助项目(81301711)
