摘要
目的：本实验建立了紫外分光光度法测定调味品中脱氢乙酸的含量.方法：样品中脱氢乙酸在酸性条件下经水蒸气蒸馏和有机溶剂萃取后,用紫外分光光度计在308nm波长处测定吸光度,与标准比较定量.结果：紫外分光光度法测定调味品中脱氢乙酸加标回收率在94.1％ ～ 103.7％之间,RSD为0.4％ ～ 4.7％.结论：该方法快速、成本低、操作简便,准确度和精密度高,可应用于生抽酱油、老抽酱油、米醋、蚝油、烧烤汁、甜辣酱等调味品中脱氢乙酸的快速测定.
Objective ： To establish a method for determination of dehydroacetic acid content in condiments by Ultraviolet spectrophotometric method. Methods ~ Dehydroacetic acid in the sample was distilled under acidic condition and extracted with organic solvent. The content was determined by ultraviolet spectrophotometer at 308 nm wavelength and content was quantitatively determined with the comparison of standard solutions. Results： The recovery was 94.1% - 103.7% with RSD of 0.4% - 4.7%. Conclusion ： This method is applicable in determination of dehydroacetic acid in soy sauce, vinegar, oyster sauce, barbecue juice and seasoning sauce.
出处
《中国食品添加剂》
CAS
北大核心
2015年第4期195-198,共4页
China Food Additives
关键词
紫外分光光度法
调味品
脱氢乙酸
ultraviolet spectrophotometric method
condiments
dehydroacetic acid
