摘要
选择来自于亚蔬—世界蔬菜研究与发展中心和国家作物种质资源库20个对辣椒疫病具有不同抗性的辣椒品系,采用灌根法人工接种辣椒疫霉菌3号生理小种,根据不同辣椒品系的病情指数,将20个品系划分为免疫(I)、高抗(HR)、抗(R)、中抗(MR)和感(S)5种抗病类型。采用常规方法测定辣椒叶片6种防御酶活性和根际8种土壤酶活性,判断各项指标与辣椒抗疫病特性的相关性。结果表明:叶片防御酶中苯丙氨酸解氨酶、多酚氧化酶、β-1,3-葡聚糖酶活性与植株抗病性极显著正相关,过氧化物酶活性与植株抗病性呈显著正相关,根际土壤酶中多酚氧化酶、过氧化氢酶、脲酶、蛋白酶活性与植株抗病性显著正相关。叶片中苯丙氨酸解氨酶、多酚氧化酶、β-1,3-葡聚糖酶活性可作为辣椒抗病种质资源筛选的判断依据,与植株抗病性相关的土壤酶可以作为土壤微环境调控的依据。
Twenty lines of pepper with different phytophthora-resistance were observed from AVRDC and Institute of Crop Sciences, CAAS. Race 3 with Phytophthora capsici was artificially inoculated by root-irrigation and according to disease index, the 20 lines were divided into 5 phytophthora-re-sistant types, immunity （ I） , high resistance（ HR） , resistance（ R） , moderate resistance（ MR） and susceptibility （ S） . By conventional methods, activities of 6 defense enzymes in leaf and 8 soil en-zymes in rhizosphere were measured to analyze the correlation of different indices with pepper Phyto-phthora-resistance. Activities of PAL, PPO andβ-1,3-glucanase in leaf defense enzymes had signif-icantly positive correlation with resistance. Activities of POD were positively correlated with resist-ance. Meanwhile, resistance was positively correlated with activities of PPO, CAT, Urease and Pro-tease in rhizosphere soil enzymes. Activities of PAL, PPO and β-1,3-glucanase in leaf could be used to identify the resistance of pepper. Soil enzymes related to phytophthora-resistance could be used as basis for regulating soil environment.
出处
《吉林农业大学学报》
CAS
CSCD
北大核心
2015年第2期153-159,共7页
Journal of Jilin Agricultural University
基金
辽宁省农业领域青年科技创新人才培养计划项目(2014031)
关键词
辣椒
疫病
防御酶
土壤酶
pepper
Phytophthora capsici
defense enzyme
soil enzyme
