摘要
目的：建立同时测定神农茶颗粒中夏佛塔苷、异夏佛塔苷、去氧地胆草内酯、4,5-二咖啡酰奎宁酸4个成分的高效液相色谱测定方法。方法：采用Hypersil C18色谱柱（200 mm ×4.6 mm,5μm）；以乙腈-0.025 mol·L-1磷酸溶液为流动相进行梯度洗脱,体积流量：1.3 ml·min-1,检测波长：270 nm（夏佛塔苷和异夏佛塔苷）、208 nm（去氧地胆草内酯）、327 nm（4,5-二咖啡酰奎宁酸）,柱温为室温。结果：夏佛塔苷、异夏佛塔苷、去氧地胆草内酯和4,5-二咖啡酰奎宁酸分别在5.850~117.000,4.650~93.000,4.160~83.200,5.470~109.400μg · ml-1范围内线性良好, r 值均大于0.999,平均加样回收率分别为97.70%、96.87%、97.53%、99.29%,RSD分别为1.40%、1.13%、1.69%、1.01%（n=6）。结论：该方法简便,结果准确,可用于神农茶颗粒的含量测定。
Objective：To establish an HPLC method for determining four constituents （ schaftoside, isoschaftoside, deoxyelephan-topin and 4,5-dicaffeoylquinic acid） in Shennongcha granules. Methods：An HPLC method was performed on a Hypersil C18 column （200 mm × 4. 6 mm,5 μm） with the mobile phase of acetonitrile as the phase A and 0. 025 mol·L-1 phosphoric acid solution as the phase B with gradient elution. The flow rate was 1. 3 ml·min-1 . The detection wavelength was set at 270 nm for schaftoside and isos-chaftoside, 208 nm for deoxyelephantopin and 327 nm for 4, 5-dicaffeoylquinic acid. The column temperature was room temperature. Results：The calibration curve was linear over the concentration range of 5. 850-117. 000 μg·ml-1 for schaftoside, 4. 650-93. 000 μg ·ml-1 for isoschaftoside, 4. 160-83. 200 μg · ml-1 for deoxyelephantopin and 5. 470-109. 400 μg · ml-1 for 4, 5-dicaffeoylquinic acid. The correlation coefficient of all curves was more than 0.999. The average recoverywas 97.70% （RSD=1.40%）, 96.87%（RSD=1.13%）, 97.53%（RSD =1.69%） and 99.29%（RSD =1.01%） （n =6） , respectively. Conclusion： The developed HPLC method is simple,accurate,and can be used in the content determination of Shennongcha granules.
出处
《中国药师》
CAS
2015年第5期741-744,共4页
China Pharmacist
