摘要
AIM: Esophageal cancer remains a significant healthiproblem worldwide. It is important to investigate alterationsin expression of retinoic acid receptor-β, p53 and Ki67proteins in esophageal carcinogenesis.METHODS: To find biomarkers for early identification ofesophageal cancer, we analyzed the retinoic acid receptor-j3,p53 protein and the proliferation marker Ki67 in surgicalspecimens of normal, mildly, and severely dysplastic andmalignant esophageal tissues by in situ hybridization ofRNA and immunohistochemistry.RESULTS: RAR-β was expressed in 94.3 %(33/35) of normalmucosae, 67.8 %(19/28) of the mild, 58.1% (18/31) of thesevere ieaions and 53.2 % (116/218) of tumor samples. RAR-β mRNA was expressed in 62.7 % (42/67), 55.1% (43/78) and29.2 % (7/24) of well, moderated and poorly differentiatedSSCs. The p53 and Ki67 proteins were 5.9 % (2/34) of thenormal mucosa. P53 and Ki67 stained positively in 10.7 % (3/28) and 21.4 % (6/28) of mild dysplasia, and 51.6 %(16/31)and 58. 1% (18/31) of severely dysplasia respectively.Samples from esophageal cancer showed no higher levers ofp53 and Ki67 expression than seen in severely dysplasticlesions. There was significant difference of RAR-β、 p53 andKi67 expression between normal mucosa and dysplatictissue or esophageal cancer.CONCLUSION: Loss of RAR-β expression and acctnulation ofp53 and Ki67 proteins may serve as biomarkers for earlyidentification of esophageal cancer in the high-riskpopulations.
AIM:Esophageal cancer remains a significant health problem worldwide.It is important to investigate alterations in expression of retinoic acid receptor-β,p53 and Ki67 proteins in esophageal caminogenesis. METHODS:To find biomarkers for early identification of esophageal cancer,we analyzed the retinoic acid receptor-β, p53 protein and the proliferation marker Ki67 in surgical specimens of normal,mildly,and severely dysplastic and malignant esophageal tissues by in situ hybridization of RNA and immunohistochemistry. RESULTS:RAR-β was expressed in 94.3 %(33/35)of normal mucosae,67.8 %(19/28)of the mild,58.1%(18/31)of the severe lesions and 53.2 %(116/218)of tumor samples.RAR- mRNA was expressed in 62.7 %(42/67),55.1%(43/78)and 29.2 %(7/24)of well,moderated and poorly differentiated SSCs.The p53 and Ki67 proteins were 5.9 %(2/34)of the normal mucosa.P53 and Ki67 stained positively in 10.7 %(3/ 28)and 21.4 %(6/28)of mild dysplasia,and 51.6 %(16/31) and 58.1%(18/31)of severely dysplasia respectively. Samples from esophageal cancer showed no higher levers of p53 and Ki67 expression than seen in severely dysplastic lesions.There was significant difference of RAR-β,p53 and Ki67 expression between normal mucosa and dysplatic tissue or esophageal cancer.CONCLUSION:Loss of Rar-βexpression abd accumulation of p53 and Ki67 proteins may serve as biomarkes for early identification of cancer in the high-risk populations.
作者
Min Xu Sheng-Zu Chen,Department of Nuclear Medcine,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 10002,1,China Yu-Lan Jin Jun Fu Hong Huang Ping Qu Hai-Mei Tian ZhaoYang Liu Wei Zhang,Central Laboratory for Tumor Biology,Cancer Hospital(Institute),Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100021,China
