摘要
目的研究甘遂醋炙前后3种三萜类成分(大戟二烯醇、kansenone、11-oxo-kansenonol)含有量变化及其对肠上皮细胞的毒性。方法采用HPLC法分别在210、255、270 nm测定甘遂和醋甘遂饮片中该3种成分的量。采用MTT法检测3种成分对大鼠小肠隐窝上皮细胞IEC-6细胞增殖的影响。结果醋炙后大戟二烯醇、kansenone、11-oxokansenonol含有量均降低,降低率分别为13.43%、15.15%、14.79%。对IEC-6细胞的IC50分别为71.41、15.27和14.53μmol/L。结论甘遂醋炙后3种三萜类成分含有量的降低与醋炙减毒有一定的相关性。
AIM To study the content changes of euphol,kansenone and 11-oxo-kansenonol from Kansui Radix before and after processing with vinegar and their toxicity to intestinal epithelial cells. METHODS The assay of triterpenes in processing Kansui Radix with vinegar was measured at 210 nm,255 nm and 270 nm by HPLC,respectively. The MTT assay was employed to examine the growth inhibition of IEC-6 cells which were pretreated with these triterpenes. RESULTS The contents of the three kinds of triterpenes decreased during the processing,and the reduction rates were 13. 43%,15. 15%,and 14. 79%,respectively. The IC50 values of IEC-6 cells were71. 41,15. 27,and 14. 53 μmol/L,respectively,after being pretreated with triterpenes. CONCLUSION The experimental data indicate that there is a certain correlation between the reduction in the three kinds of triterpenes＇ contents and the attenuation during processing with vinegar.
出处
《中成药》
CAS
CSCD
北大核心
2015年第5期1045-1049,共5页
Chinese Traditional Patent Medicine
基金
国家自然科学基金项目(30973940
81373972)
国家重点基础研究发展计划(973)项目(2011CB505300
2011CB505303)
江苏省"六大人才高峰"项目(2010年度)
江苏省政府留学奖学金项目(JS-2009-061)
江苏高校优势学科建设工程资助项目(ysxk-2014)
