摘要
目的:建立银翘散(YQS)不同复方配伍组及君(J),臣(C),佐(Z),使(S)关系的HPLC指纹图谱,找出配伍前后物质基础变化及复方配伍过程中物质基础相互作用关系,从整体上探讨YQS复方配伍关系。方法:利用Ulti Mate 3000 XRS型超快速液相色谱仪,Thermo C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-0.05%磷酸水梯度洗脱,检测波长237 nm,流速1 m L·min-1,对YQS及君臣佐(J,C,Z),君臣使(J,C,S),君佐使(J,Z,S),君臣(J,C),J,C,Z,S不同配伍关系进行HPLC指纹图谱研究;利用指纹图谱处理软件对各组间HPLC指纹图谱进行数据分析。结果:YQS与各配伍组相似度由高到低为J,C,S(0.981)>J,C(0.959)>J,C,Z(0.953)>J,Z,S(0.806);YQS与J,C,Z,S组相似度由高到低为J(0.749)>C(0.573)>Z(0.136)>S(0.104)。YQS配伍合煎与单煎相加后的指纹图谱相比,14个峰面积降低,63个峰面积增加。结论:君臣药是YQS物质基础主要来源;本文从物质基础组成的角度,对YQS中J,C,Z,S在全方中的重要性进行了数据分析,使传统方解更具有说服力。
Objective： To find out the changes before and after the material basis of compatibility, and to discover the interaction among the process of compound mixing, through established the HPLC fingerprints of Yinqiao San （YQS） and Jun （J）, Chen （C）, Zuo （Z）, Shi （S）. Then investigated the relationship of compound compatibility of YQs on the whole. Method： The chromatographic condition was： Ultimate 3000 XRS ultra fast liquid chromatography, thermo ClS （4. 6 mm ×250 mm, 5 μm） column, methanol-0. 05% phosphoric acid water as the mobile phase gradient elution, the detection wavelength was 237 nm and flow rate was 1 mL .min-1 Researched and established the HPLC fingerprint of YQS and other groups, then used the fingerprint processing software to analys the data of each group HPLC fingerprint. Result： The similarities from high to low between YQS and other compatibility groups are ： J, C, S （0.981） 〉J, C （0.959） 〉J, C, Z （0.953） 〉J, Z, S （0.806）. The similarities from high to low between YQS and J, C, Z, S are： J （0.749） 〉C （0.573） 〉Z （0.136） 〉S （0. 104）. Compared with the single decoction added, the fingerprint areas of YQS decoction had 14 peaks decreased and 63 peaks increased. Conclusion： J and C were the main source of material basis of YQS. In this paper, from the perspective of the material basis composition, the datas analysed how important of J, C, Z and S in the whole prescription. This result made the traditional interpretation of prescription more persuasive.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2015年第10期47-50,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81230089)
山东省自然科学基金项目(ZR2014HQ046)
关键词
银翘散
复方配伍
君臣佐使
指纹图谱
Yinqiao San
compound compatibility
Jun Chen Zuo Shi
fingerprint
