摘要
目的:探讨人参皂苷化合物K(简称人参皂苷CK)对顺铂所致小鼠急性肾损伤的保护作用。方法:32只小鼠随机分为4组,分别为正常组、模型组、人参皂苷CK低、高剂量组(20,40 mg/kg)。除正常组别外,其他组别小鼠第5 d给药后1 h单次腹腔注射顺铂10 mg/kg,给药组分别给予人参皂苷CK 20 mg/kg、40 mg/kg连续灌胃10 d;正常组和模型组给予等体积生理盐水。第10d测定小鼠血清肌酐(Scr)和尿素氮(BUN)含量,检测肾脏组织超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)水平,HE染色切片观察肾脏组织病理学变化。结果:与正常组相比,模型组小鼠血清Scr、BUN和MDA含量明显上升,SOD和GSH含量显著下降,病理改变加重,肾小管上皮细胞凋亡;人参皂苷CK给药组20、40mg/kg均可改善小鼠肾脏组织形态,抑制SOD和GSH活性下降,降低MDA含量。人参皂苷CK 20mg/kg给药组小鼠BUN、Scr含量均显著升高,分别为(6.4±0.7mmol/L)、(30.8±1.9μmol/L);40mg/kg给药组BUN水平显著升高,为(6.4±0.4mmol/L)。结论:人参皂苷CK对顺铂所致小鼠急性肾损伤有明显保护作用。
Objective： To investigate the ginsenoside compand K protective effects against cisplatin-induced acute kidney injury in mice. Methods：Thirty-two mice were randomly divided into four groups with 8 rats each group,which were normal group,model group,ginsenoside CK low and high dose groups（ 20,40 mg / kg）. Other three groups were given CDDP intraperitoneally of 10 mg / kg,single dose at the 5th day,1h after administration except control group,treatment groups were given Ginsenoside CK 20 mg / kg,40 mg / kg intragastrically,once daily for 10days; the normal group and model group were given an equal volume of saline. The contents of serum creatinine（ Scr）,blood urea nitrogen（ BUN） and the level of superoxide dismutase（ SOD）,malondialdehyde（ MDA） and glutathione（ GSH） were measured after 10 days of administration,HE staining was used to observe the pathological changes. Results： Compared with the normal group,contents of Scr,BUN in serum and urine content of MDA were significantly decreased,and the activity of SOD and GSH was significantly increased in the model group,renal tubular necrosis; Ginsenosides CK treatment group 20 mg / kg and 40 mg / kg can improved mouse kidney tissue morphology,inhibiting the activity of SOD and GSH decreased,decreasing MDA content. Ginsenoside CK 20 mg / kg pretreatment markedly decreased the serum levels of BUN and Scr（ 6. 4 ± 0. 7mmol / L）,（ 30. 8 ± 1. 9μmol / L）; while the content of BUN was significantly increased in Ginsenoside CK 40 mg / kg pretreatment group（ 6. 4 ± 0. 4mmol / L）. Conclusion： Ginsenoside CK can effectively inhibiting the acute kidney injury in mice induced by cisplatin.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2015年第1期44-46,共3页
Pharmacology and Clinics of Chinese Materia Medica
基金
吉林省科技发展计划项目(NO.20125064)
中国博士后面上项目(NO.2012M520483)
