摘要
目的:探讨microRNA-130a(miR-130a)在胃癌耐药细胞中的表达,并对进一步作用的机制进行研究。方法:采用体外转染法将miR-130a模拟物或抑制剂分别瞬时转染到SCG7901和SCG7901/DDP细胞后,应用Real-time PCR检测转染前后细胞中miR-130a的表达情况;CCK8实验检测转染前后细胞对顺铂(DDP)敏感性的变化;并用Western blot检测转染前后细胞中PETN的表达变化。结果:MiR-130a在SGC7901/DDP中的表达水平是SGC7901细胞的(8.33±1.21)倍(P<0.01)。SGC7901转染miR-130a mimics后,细胞中miR-130a表达水平是转染前的(5.52±1.65)倍(P<0.01),DDP对SGC7901增殖的抑制率较转染前明显下降(P<0.01),细胞内PTEN表达水平较转染前明显下降(P<0.01)。SCG7901/DDP在转染miR-130a inhibitor后,细胞中miR-130a表达水平是转染前的(0.18±0.04)倍(P<0.01),DDP对SCG7901/DDP增殖的抑制率与转染前比较明显增加(P<0.01),细胞内PTEN表达水平较转染前明显增高(P<0.01)。结论:MiR-130a通过下调PTEN的表达来调节胃癌细胞对DDP的耐药。
Objective：To investigate the regulatory effects and mechanisms of microRNA - 130a（ miR - 130a） on the resistance of gastric cancer cells. Methods：MiR - 130a mimics and miR - 130a inhibitor in vitro was transiently transferred into SCG7901 and SCG7901/DDP cells respectively. The expression level of miR - 130a was detected by RT - PCR. Cell viability was analyzed by CCK8 assay. Expression of PTEN protein was detected by Western blot. Re- suits ：The expression level of miR - 130a was up - regulated in SCG7901/DDP cells as compared with SCG7901 cells （ P 〈 0.05 ）. The relative expressionlevel of miR - 130a in miR - 130a mimics - transfected SCG7901 was significant- ly increased. Over - expression of miR - 130a inhibited cisplatin chemosensitivity, and the up - regulation of miR - 130a led to a significant decrease in the PTEN protein levels. The relative expression level of miR - 130a in miR - 130a inhibitor - transfected SCG7901/DDP was significantly decreased. Low - expression of miR - 130a promoted cisplatin chemosensitivity, and the down - regulation of miR - 130a led to a significant increase in the PTEN protein levels. Conclusion：MiR - 130a could play a role in the development of resistance in gastric cancer cells, at least in part by targeting PTEN.
出处
《现代肿瘤医学》
CAS
2015年第10期1358-1361,共4页
Journal of Modern Oncology
