摘要
目的:研究积雪草醇提物治疗2型糖尿病(T2DM)合并非酒精性脂肪性肝病(NAFLD)的作用及可能的治疗机制。方法:采用高糖高脂饮食4周+腹腔注射小剂量链脲佐菌素(30mg/kg)建立T2DM合并NAFLD模型,随机分为5组:模型对照组,二甲双胍组(75mg/kg),积雪草低(60mg/kg)、中(100mg/kg)、高(200mg/kg)剂量组;测血液及肝脏生化指标,血清胰岛素水平;观察肝脏病理学改变;检测AMPKα及p-AMPKα蛋白表达。结果:积雪草醇提物各剂量组均明显降血清中及肝脏组织中的CHO、TG水平,降低肝脏指数,改善肝功能(P<0.05,P<0.01),且形态学表现及脂肪变性程度均较模型对照组得到改善;积雪草高剂量组大鼠肝脏的AMPKα及p-AMPKα蛋白表达水平增高(P<0.05)。结论:积雪草醇提物兼具有降糖及抗脂肪肝作用,而其发挥作用的机制可能与激活AMPK的表达有关。
Objective： To evaluate the effects of the centella asiatica extract on type 2 diabetes complicated with non- alcoholic fatty liver disease （NAFLD）. Methods： Type 2 diabetes NALFD model rats was induced by intraperitoneal injection of Streptozocin （STZ, 30mg/kg） after high glucose and high fat diet for 4 weeks. After the modeling, rats were randomly divided into 5 groups： model control group, Metformin group （75mg/kg）, centella asiatica low, middle and high dose groups （60, 100, 200mg/kg, respectively）. The rats＇ body weight, food and water intake, TG and TC levels were measured weekly. While after treatment for 4 weeks, the serum level and liver tissue were measured with automatic biochemical analyzer test, the serum insulin level was detected, the morphology of rat liver was observed, and the hepatic AMPK alpha and p-AMPK alpha protein expression level were detected. Results： Compared with model control group, every dose of centella asiatica extract groups and metformin group could significantly reduce the CHO and TG level in serum and liver tissue, reduce the liver index, and improve liver function （GOP, GPT） （P〈0.05, P〈0.01）. Besides, the morphological manifestations and the degree of steatosis in the treatment group were improved compared with the model control group. Centella asiatica high-dose group still could increase the expression of AMPK alpha and p-AMPK alpha protein in the rat liver （P〈0.05）. Conclusion： Centella asiatica extract has the effects of anti-diabetes and lipid-reduction, moreover, its function mechanism maybe related to the activation of AMPK expression.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2015年第5期1524-1530,共7页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
科学技术部国家国际科技合作专项(No.2012DFG31550)
教育部中医养生学重点实验室
北京市中医养生学重点实验室
北京中医药大学创新团队项目(No.2011-CXTD-19)~~
关键词
2型糖尿病
非酒精性脂肪性肝病
积雪草醇提物
Type 2 diabetes
Non-alcoholic fatty liver disease
Centella asiatica alcohol extract
