摘要
目的以兔构建的宫腔粘连(intrauterine adhesions,IUA)模型存在兔饲养成本偏高、不利于大规模复制模型等缺点,且目前为止,白细胞介素21(Interleukin-21,IL-21)与IUA的关系鲜有报道。文中旨在构建机械和脂多糖(lipopolysaccharide,LPS)感染双重损伤法的大鼠IUA模型,并检测其血清IL-21的表达水平,探讨IL-21与IUA形成的相关性。方法将健康雌性SD大鼠40只随机分为对照组(开腹但不损伤子宫)、机械损伤组(刮取子宫内膜)、感染损伤组(子宫腔放置细菌LPS棉线48h)、双重损伤组(刮取子宫内膜后在宫腔放置细菌LPS棉线48h),每组10只。构建IUA模型后7d收集子宫组织,HE染色观察子宫内膜腺体;Masson染色检测子宫内膜纤维化程度;放射免疫法检测各组大鼠血清IL-21水平,并与子宫内膜腺体数量及子宫内膜纤维化程度进行相关性分析。结果双重损伤组子宫内膜腺体数量(3.59±1.20)较对照组(12.87±2.68)、机械损伤组(11.66±2.34)、感染损伤组(11.59±1.47)均减少,差异有统计学意义(P<0.05)。双重损伤组子宫内膜纤维化面积比(0.65±0.03)较对照组(0.25±0.04)、机械损伤组(0.30±0.07)、感染损伤组(0.32±0.08)均升高,差异有统计学意义(P<0.05)。双重损伤组血清IL-21水平[(286.21±27.80)pg/m L]较对照组[(118.65±22.55)pg/m L]、机械损伤组[(176.20±19.05)pg/m L]、感染损伤组[(187.98±16.51)pg/m L]升高,差异有统计学意义(P<0.05)。IL-21水平与子宫内膜纤维化面积比呈正相关(r=0.271,P<0.05)。结论采用机械和感染双重损伤法可以成功建立大鼠IUA模型,该大鼠模型血清IL-21水平明显升高,与子宫内膜纤维化程度呈正相关,提示IL-21可能间接参与IUA的形成。
Objective Interleukin-21（IL-21） is likely to contribute to the development of liver fibrosis, but up to now, no study has been reported on the relationship between IL-21 and intrauterine adhesions （ IUA） .This study aimed to establish a rat model of IUA induced by mechanical injury and lipopolysaccharide （LPS） infection （dual injury）, determine the expression level of serum IL-21, and confirm the association of serum IL-21 with the formation of IUA. Methods Forty healthy female SD rats were randomly divided into four groups of equal number,control, mechanical injury, LPSinfection, and dual injury.At 7 days after IUA modeling,uterine tissue-swere collected from all the animals for observation of the endometrial glands,detection of the degree of IUA by Masson staining, measure-ment of the serum IL-21 level by radioimmunoassay, and analysis of the correlation between the number of endometrial glands and the de-gree of fibrosis. Results The number of endometrial glandswas significantly smallerin the dual injury group （3.59±1.20） than in the mechanical injury （11.66±2.34） and LPSinfection group（11.59±1.47）（P＆lt;0.05）, while the proportion of fibrosis area wassignificantly higher in the former group（0.65±0.03） than in the lattertwo（0.30±0.07 and 0.32±0.08）（P＆lt;0.05）.The level of serum IL-21 was signifi-cantly increased in the dual injury group （[286.21±27.80]pg/mL） as compared with those in the control （ [ 118.65 ±22.55 ] pg/mL ） , mechanical injury（[176.20±19.05]pg/mL）, and LPS infection group （[187.98±16.51]pg/mL） （P〈0.05）, with a positive correlation be-tween the IL-21 level and theproportion of fibrosis area （ r=0.271, P〈0.05） . Conclusion A rat model ofIUAwas successfully established by mechanical injury and lipopolysaccharide （LPS） infection.The evi-dent increase of serum IL-21 in the IUA model was positively correlated with the percentage of fibrosis area, suggesting that IL-21 may be involved indirectly in the formation of IUA.
出处
《医学研究生学报》
CAS
北大核心
2015年第4期346-349,共4页
Journal of Medical Postgraduates
基金
国家自然科学基金(81270658)
广州市科技计划项目(11C32120702)
