摘要
目的通过电转染介导转化生长因子β1(TGF-β1)转染兔骨髓间充质干细胞(BMSCs),观察Ⅱ型胶原表达的情况。方法用全骨髓细胞贴壁培养法分离、培养兔BMSCs;诱导14 d后,免疫组化和Western blot法检测Ⅱ型胶原表达。结果 BMSCs CD90表达阳性,CD31表达阴性;成功转染TGF-β1至BMSCs;通过免疫组化及Western blot法检测TGF-β1组细胞内Ⅱ型胶原有较强的表达,与增强型绿色荧光蛋白(EGFP)组和空白对照组相比差异有统计学意义(P<0.05)。结论电转TGF-β1质粒至兔BMSCs,可以促进Ⅱ型胶原表达。
Objective To observe cases of type Ⅱ collagen expression after transforming growth factor-β1 （TGF-β1） infected in rabbit bone mesenchymal stem cells（ BMSCs） by electropration. Methods BMSCs from rabbit were isolated and cultured. 14 days after induction, immunohistochemistry and Western blot methods type Ⅱ colla-gen were used to detect the expression of type Ⅱ collagen. Results CD90 was positive and CD31 was negative by flow cytometry;successfully transfected with TGF-β1 to the BMSCs,the strong expression of type Ⅱ collagen in TGF-β1 group cells was shown by Western blot and immunocy-tochemical stain, which was compared with empty plasmid group and the control group,and the difference was statistically significant（P 〈 0. 05）. Conclusion TGF-β1 plasmid by electroporation to rabbit BMSCs can facilitate expression of collagen Ⅱ.
出处
《安徽医科大学学报》
CAS
北大核心
2015年第5期581-584,共4页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:31260233)
关键词
TGF-Β1
骨髓间充质干细胞
电转染
Ⅱ型胶原
transforming growth factor beta 1
mesenchymal stem cells
electropration
collagen Ⅱ
