摘要
目的通过检测miRNA-141、miRNA-145在非小细胞肺癌中的表达,探讨其与非小细胞肺癌临床病理的关系。方法采用Real time-PCR法对48例非小细胞肺癌组织及正常肺组织的miRNA-141、miRNA-145进行定量分析,结果由2(-△△CT)处理,并分析与临床病理资料的关系。结果 1NSCLC患者肿瘤组织中miRNA-141表达水平明显升高,而且其表达与临床分期、病理类型显著相关(P<0.05),miRNA-141在鳞癌患者肿瘤组织表达高于腺癌(P<0.05),而在不同年龄、性别、肿瘤大小患者间差异无统计学意义(P>0.05)。2NSCLC患者肿瘤组织中miRNA-145表达水平明显下降,而且其表达与临床分期、病理类型显著相关(P<0.05),miRNA-145在腺癌患者肿瘤组织表达低于鳞癌(P<0.05),而在不同年龄、性别、肿瘤大小患者间差异无统计学意义(P>0.05)。结论 miRNA-141高表达及miRNA-145低表达与非小细胞肺癌的临床分期、病理分级密切相关,miRNA-141、miRNA145有可能作为非小细胞肺癌的重要肿瘤标志物。
Objective To detect the expressions of miRNA-141 and miRNA-145 in non-small cell lung cancer （NSCLC）and explore their relationship with the clinical pathology of NSCLC.Methods We made a quantitative analysis of miRNA-141 and miRNA-145 expressions in 48 cases of NSCLC tissues and adjacent normal tissues by real time-PCR.Then 2 （-△△CT） method was used to analyze their relationship with the clinicopathologic data.Results ① miRNA-141 expression was significantly increased in tumor tissue of NSCLC patients,and it also showed a significant correlation with clinical staging and pathological type （P 〈0.05）.The expression of miRNA-141 in the tumor tissue in the patients with squamous carcinoma was higher than that in the patients with adenocarcinoma （P 〈0.05）,but there was no significance difference in age,gender,tumor size or smoking history of the patients （P 〉0.05）.② miRNA-145 expression was significantly decreased in the tumor tissue of NSCLC patients,and it also had a significant correlation with clinical staging and pathological type （P 〈0.05）.The expression of miRNA-141 in the tumor tissue of the patients with adenocarcinoma was lower than that in the patients with squamous carcinoma （P 〈0.05）.However,no significance difference was found in age,gender,tumor size or smoking history of the patients （P 〉0.05）.Conclusion High expression of miRNA-141 and low expression of miRNA-145 are closely related to clinical staging and pathological type of NSCLC.miRNA-141 and miRNA-145 have the potential to be used as one of valuable tumor markers of NSCLC.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2015年第3期368-372,共5页
Journal of Xi’an Jiaotong University（Medical Sciences）
基金
陕西省科技攻关项目资助(No.2012K13-02)~~
