摘要
目的探讨宫颈上皮内瘤变(CIN)、宫颈鳞癌组织中组蛋白去乙酰化酶1(HDAC1)的异常表达以及CIN、宫颈鳞癌发病与HPV16/18感染的相关性。方法应用原位杂交(ISH)技术及免疫组织化学(SP)方法检测慢性宫颈炎组织(慢性宫颈炎组)、CIN组织(CINⅠ组、CINⅡ组、CINⅢ组)和宫颈鳞癌组织(宫颈癌组)中HPV16/18感染以及HDAC1的表达。结果慢性宫颈炎组、CINⅠ组、CINⅡ组、CINⅢ组和宫颈癌组中HPV16/18的感染率分别为8.33%、34.78%、48.00%、65.21%、78.00%,组间差异有统计学意义(P<0.05);HDAC1阳性表达率分别为0、13.04%、32.00%、47.82%和76.00%,组间差异有统计学意义(P<0.05)。HPV16/18感染组的HDAC1阳性表达率高于HPV16/18未感染组(P<0.05)。HDAC1的阳性表达与宫颈癌的临床分期无关(P>0.05),而与组织分化、间质浸润深度及盆腔淋巴结转移相关(P均<0.05)。结论在宫颈癌的发生发展中,HPV16/18感染可能是通过提高组蛋白去乙酰化酶1的水平抑制转录而发挥作用。
Objective To explore the anomalous expression of HDAC1 in cervical intraepithelial neoplasia（CIN）and cervical carcinoma and its relationship with the infection of HPV16/18. Methods The expressions of HDAC1 protein and HDAC1 infection were detected by in situ hybridiza-tion（ISH）and immunohistochemistry staining（SP）in tissues from patient with chronic cervicitis（the chronic cervicitis group）,CIN（the CINⅠgroup,the CINⅡgroup and the CINⅢgroup）and cervical squamous carcinoma（the cervical carcinoma group）. Results The infection rate of HPV16/18 was 8.33%,34.78%,48.00%,65.21%,78.00%in patients with chronic cervicitis,CINⅠ,CINⅡ,CINⅢand cervical carcinoma re-spectively. There was significant difference between groups（P〈0.05）. The positive expression rate of HDAC1 was 0,13.04%,32.00%,47.82%and 76.00%in chronic cervicitis,CINⅠ,CINⅡ,CINⅢand cervical carcinoma,respectively. The differences were statistically significant（P〈0.05）. The positive expression of HDAC1 in the HPV16/18 infected patients was significantly higher than those uninfected with HPV16/18（P〈0.05）. The HDAC1 positive expression had no relationship with clinical stages of cervical carcinoma,however,it was related to the tissue differentia-tion,stromal invasion and pelvic lymph node metastasis（all P〈0.05）. Conclusion The infection of HPV16/18 may play an important role in the carcinongenesis of SCC through increasing the expression of HDAC1.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2015年第5期464-467,471,共5页
Journal of China Medical University
关键词
组蛋白去乙酰化酶1
HPV宫颈上皮内瘤变
宫颈癌
原位杂交
histone deacetylase 1
HPV
cervical intraepithelial neoplasia
cervical carcinoma
in situ hybridization
