摘要
目的:探讨低温下鄂尔多斯高原碱湖钝顶螺旋藻(S1)PM H+-ATPase活性的变化及其与抗低温的关系,并与非洲Chad湖钝顶螺旋藻(S2)进行比较。方法:以鄂尔多斯钝顶螺旋藻(S1)和非洲Chad湖钝顶螺旋藻(S2)为材料,采用两相法提纯质膜(plasms membrae PM),钼蓝法测定PM H+-ATPase活性。结果:结果显示:低温直接处理和锻炼后低温处理,S1和S2PM H+-ATPase活性都是先升后降。S1PM H+-ATPase活性比S2PM H+-ATPase活性稳定。低温锻炼可提高PM H+-ATPase活性。PM H+-ATPase活性变化范围S1<S2。低温直接处理,S1的生理活动临界低温为0℃,S2的生理活动临界低温为10℃;锻炼后处理,S1的生理活动临界低温为0℃,S2的生理活动临界低温为5℃。结论:S1对低温有较强的适应性。
Objective：To explore the variation of PM H＋-ATPase of the Spirulina platensis（ S1 ） from alkaline lake in Erdos Plateau and its relationship with low temperature resistance under low temperatures stress, and comparing them with that of S. platensis （ S2 ） from Chad Lake in Africa at the same time. Methods： After the purification of the plasma membranes of both Spirulina platensis （ S1 ） from alkaline lake in Erdos Plateau and S. platensis （ S2 ） from Chad Lake in Africa with aqueous polymer two -phase partitioning system, the activities of PM H＋ -ATPase are determined by Mo- Bluecolor method. Results：It is discovered that they being exposed right to low temperature or after exercised under low temperature,as temperature dropping and days lasting, the activities of PM H＋ -ATPase of both S1 and S2 rise first, and drop afterward. The stability of PM H＋-ATPase activity of S1 is better than that of S2 at the same conditions above. The activity of PM H＋-ATPase can be improved through low temperature exercise. The altering range of PM H＋-ATPase activities is S1 〈S2. They exposed right to low temperatures,the critical low temperature of the physiological activity of S1 is 0℃, S2 10℃, while it is 0℃ for S1 ,5℃ for S2 after exercised under low temperatures. Conclusion： S~ has better adaptability to low temperature.
出处
《内蒙古医科大学学报》
2015年第1期1-5,共5页
Journal of Inner Mongolia Medical University
基金
国家自然科学基金项目(30460104)
内蒙古自然科学基金项目(200308020310)
内蒙古高等学校科学研究项目(NJ04036)
