摘要
目的观察短期模拟微重力对新生乳鼠大脑皮层及小脑的原代细胞的凋亡率及一氧化氮合酶活性的影响。方法取新生1天SD乳鼠的皮层及小脑两个脑区,做原代细胞培养,将培养物随机分为模拟微重力组和地面对照组。回转一天后,用流式细胞仪检测其细胞凋亡情况及一氧化氮合成酶(NOS)活性的变化。结果流式细胞仪结果显示皮层和小脑的原代细胞在经过一天的模拟微重力刺激后,其细胞凋亡率与对照组相比无显著差异,皮层与小脑相比,皮层对模拟微重力稍敏感。一氧化氮合成酶活性在经过模拟微重力刺激以后也无显著变化,且两个脑区之间也无明显变化。结论经过一天的模拟微重力后,皮层和小脑原代细胞的凋亡率和一氧化氮合成酶活性均无显著变化,但两个脑区的变化趋势稍有差异,说明皮层和小脑对模拟微重力的响应稍有不同。
Objective To investigate effects of 1-day simulated microgravity on apoptosis and activity of NOS of primary cells from cortex and cerebellum of SD neonatal rats. Methods Primary cells were obtained from cortex and cerebellum of SD neonatal rats. After cultured for 7 days, primary cells of the two brain areas were randomly divided into control group and simulated microgravity group, The microgravity group was treated with clinostat for l day and control group was cultured in the same incubator. After that, flow cytometry was carried out to investigate apoptosis and activity of NOS of the two kinds of primary cells. Results Apoptosis and activity of NOS of primary cells from the two brain areas showed no difference between simulated microgravity group and control group. But there were fine differences in apoptosis between the changes of cortex and cerebellum. Conclusion Our data indicated that apoptosis and activity of NOS of primary cells from cortex and cerebellum seemed not to be affected by 1-day simulated microgravity, but the responses of cortex and cerebellum to simulated microgravity had a little difference from each other.
出处
《生命科学仪器》
2015年第2期35-38,共4页
Life Science Instruments
基金
国家重大科学仪器设备开发专项(2012YQ04014008
2013YQ03059514)
