摘要
以人工选育的浆蜂为材料,提取每个工蜂样品的基因组DNA,对csd基因3区进行PCR扩增、克隆和测序,最终获得了13个浆蜂csd基因单倍型。对浆蜂与原种意大利蜜蜂csd基因的多态性进行比较。结果表明浆蜂和原种意大利蜜蜂的核苷酸多样度(π)分别为0.057 85±0.004 92和0.043 80±0.005 75,两者之间没有显著差异。系统进化树表明来自浆蜂和原种意大利蜜蜂的单倍型混杂在一起,没有形成完全独立的2个分支。群体分析表明浆蜂和原种意大利蜜蜂之间的Fst距离是0.036 9,两者之间的遗传分化很弱。这些结果说明人工选育对浆蜂csd基因的多态性没有产生显著影响。
In this study, artificially bred high jelly producing honeybees were used as the experimental material.Genome DNA was extracted from each honeybee sample for PCR amplification of the csd region 3 ,PCR products were cloned and sequenced.Finally 13 csd haplotypes were obtained.The difference in the polymorphism of csd gene between high royal jelly producing honeybee and native Italy honeybee was compared. The results showed that the nucleotide diversity （π） values of csd in these two strains aide 0.057 85±0.004 92 and 0.043 80±0.005 75 ,respectively.Z test indicated that there is no significant difference between the π values of these two strains.Phylogenetic tree showed that csd haplotypes do not form two branches reflecting the two strains.Rather, they are well mixed among each other.The Fst distance between high royal jelly producing honeybee and native Italy honeybee is 0.036 9,indicating a weak genetic differentiation between these two strains.These results indicated that artificial selection has no effect on the polymorphism of csd gene in high royal jelly producing honeybees.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2015年第2期323-327,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
国家自然科学基金项目(31260584)
国家蜂产业技术体系资助项目(CARS-45-kxj12)
江西省自然科学基金项目(20114BAB214001)
关键词
蜜蜂
csd
多态性
单倍型
遗传分化
honeybee
csd
polymorphism
haplotype
genetic differentiation
