摘要
从江西红壤花生地中，筛选出3株产吲哚乙酸（IAA）菌株，其中ZH5菌株具有较强分泌IAA的能力且性能稳定，培养24h时IAA产量达到43．18μg／ml，且菌株ZH5具有较强的解磷能力。通过菌株形态、生理生化特征测定及16SrRNA的保守序列鉴定，初步确定菌株ZH5为巨大芽孢杆菌（Bacillusmegaterium）。对菌株生长和发酵条件进行研究，结果表明促进菌株ZH5生长和增强分泌IAA的最佳培养条件基本一致，最佳培养条件初始pH为6～8，最佳通气量为50ml／250ml，30℃摇床培养24h；最佳碳源和氮源分别是蔗糖和酵母粉。在实验室中进行盆栽试验，结果表明用ZH5菌株处理的花生株高增加了54．0％；植株鲜重增加了49．8％；并且ZH5菌株处理的花生植株全磷及花生根长、根尖数、根表面积及根体积均有显著增加，分别增加了21．84％、64．20％、28．20％、34．35％和68．67％。该研究结果为菌株ZH5在农业生产中的应用提供了理论依据和实验基础。
Three IAA-producing strains were isolated from the peanuts growing in red soil. Among the three strains, ZH5 showed the highest and most stable IAA excreting ability （43.18 μg/ml in 24 h cultivation）, combining additional phosphate-dissolving capability as well. Based on morphological, part of the physiological and biochemical characteristics and 16S rRNA sequences analysis, it was identified as Bacillus megaterium. Single factor tests were designed to explore conditions optimal to growth and fermentation of the strain. Results showed that the optimum condition for growth was consistent with that for excretion, the optimal culturing conditions for both growth and IAA secretion were pH 6-8, filling volume 50 ml/250 ml flask, incubation at 30℃ for 24 h, the best carbon and nitrogen sources were mannit and yeast extract. These results provided the experimental basis about the strains for the development of bio-organic fertilizer in the future.
出处
《土壤》
CAS
CSCD
北大核心
2015年第2期361-368,共8页
Soils
基金
国家科技支撑计划课题项目(2011BAD41B01)资助
关键词
红壤
植物促生菌
IAA
促生
解磷
Red soil
PGPB
Indole-3-acetic acid （IAA）
Phosphate-dissolving
Growth-promoting
