摘要
目的比较全身型重症肌无力(generalized myasthenia gravis,GMG)患者异常胸腺(胸腺瘤、胸腺增生)与正常胸腺microRNA(miRNA)表达谱的差异,以探讨胸腺miRNA在重症肌无力发病机制中的作用。方法应用RiboArrayTMmiRNA芯片检测技术分别比较GMG伴胸腺瘤(4例)、胸腺增生(4例)患者胸腺与正常对照(4例)胸腺miRNA表达谱的差异,并利用生物信息学预测可能受其调控的靶基因。结果 GMG胸腺瘤组、GMG胸腺增生组与正常对照组之间胸腺miRNA聚类分析树状图差异明显;GMG胸腺瘤组较正常对照组显著差异表达的胸腺miRNA共有16个,其中14个下调,下调最显著的为hsa-miR-362-3p,2个上调,上调最显著的为hsa-miR-125a-5p;GMG胸腺增生组较正常对照组显著差异表达的miRNA共有38个,其中下调者37个,下调最显著的为hsa-miR-548av-3p,上调者1个,为hsa-miR-4442。结论异常表达的胸腺miRNA可能在重症肌无力发病中起作用。
Objective To compare the expression of miRNAs between the abnormal thymus of generalized myasthenia gravis （GMG） and normal thymus tissue, and to develop primary understanding of the pathogenesis of GMG. Methods We investigated 8 thymus （4 thymomas and 4 thymic hyperplasias） of myasthenia gravis samples to examine miRNA expression profiles through miRNA microarray compared to 4 normal thymus. We also predicted the possible targets of miRNA by bioinformatics. Results The miRNA microarray chip analysis identified 16 miRNAs differentially expressed in thymomas of GMG compared with the health control group, 14 miRNAs were down-regulated and 2 up-regulated. In comparison between thymie hyperplasias GMG and the health control groups, 37 miRNAs are down-regulated, while 1 miRNA are up- regulated in the hyperplastic GMG group. Conclusions There is a specific miRNA profile in abnormal thymus （thymomas and thymic hyperplasia） of GMG comparing to normal thymus tissue. MiRNA might play an important role in the pathogenesis of GMG.
出处
《中国神经免疫学和神经病学杂志》
CAS
2015年第3期171-176,181,共7页
Chinese Journal of Neuroimmunology and Neurology
基金
广西自然科学基金项目资助项目(2014GXNSFAA118262)
广西壮族自治区卫生厅科研资助项目(Z2007084
Z2011047)
关键词
重症肌无力
胸腺瘤
胸腺增生
微RNAS
芯片分析技术
基因表达谱
myasthenia gravis
thymoma
thymus hyperplasia
microRNAs
microchip analyticalprocedures
gene expression profiling
