摘要
以5个糜子栽培生态区的40份糜子种质资源为试材,利用5个糜子特异性SSR标记对试材进行遗传多样性分析,旨在为糜子核心种质的构建和筛选提供一定的理论基础.结果表明：40份材料中共检测出15个等位基因变异,平均每个位点3个;40份材料间的遗传相似系数为0.692～0.726;聚类分析将试材分为5类,分别位于东北春糜子区、华北夏糜子区、北方春糜子区、黄土高原春夏糜子区和南方秋冬糜子区.同时,测定了以上试材的形态和生理指标（株高、主穗长和叶绿素含量）,发现试材间存在多样性：（1）40个糜子材料的旗叶叶绿素含量介于27.23～43.10 g·L-1之间,倒二叶叶绿素含量介于17.00～41.17 g·L-1之间,其中185号材料（红糜子）叶绿素含量均最大.（2）40份材料的株高介于119.73～273.97 cm之间;其中,60号材料（镇原大糜子）最高,为273.97 cm,247号材料（黑糜子）最低,为119.73 cm;（3）主穗长介于17.00～66.07 cm;其中257号材料（黑糜子）最长,为66.07 cm,265号材料（鹅头）最短,为17.00 cm.通过对糜子材料部分形态、生理指标及5对糜子特异性SSR标记分析了遗传多样性,从聚类结果得出,SSR标记出的糜子多态性表现出了一定的地域性.
Using 40 broomcorn millet germplasm resources as material,the genetic diversity was analyzed based on 5 simple sequence repeat （SSR） markers,to provide certain theoretical basis for the construction of core germplasm corn millet and filtering.The results showed：15 alleles were detected among the materials,3 alleles were the average per locus.Genetic similarity coefficient among 40 accessions ranged from 0.692～0.726,which were classified into five categories including North China Spring Millet area,Summer millet area,Northern spring millet area in the Loess Plateau,the Spring and Summer millet zone and the South autumn and winter millet area,respectively.Meanwhile,the morphology and physiological indexes （plant height,ear length and the chlorophyll content）of the material were assayed.The results showed：（1） The chlorophyll content of flag leaf of the 40 accessions ranged from 27.23 g · L-1 to 43.10 g ·L-1,the chlorophyll content of blade in the second leaf from top ranged from 27.93 g · L-1 to 41.17 g · L-1;among them the accession of No.185 （Hong mizi） was the biggest.（2） The plant height of the 40 accessions were ranged from 119.73 cm to 273.97 cm,among them,Accession 60 and 247 were the highest and the lowest,respectively;（3） The panicle length of main spike among all the accessions were ranged from 17.00 cm to 66.07 cm,257 and 265 were the longest and the shortest,respectively.Analyzed the genetic diversity through some of the physiological indicators and five pairs of millet specific SSR markers of millet material.Derived from the clustering results,SSR markers out of millet polymorphism showed some regional.
出处
《山西农业大学学报（自然科学版）》
CAS
2015年第3期225-231,共7页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
国家自然科学基金(31271791)
关键词
糜子
亲缘关系
SSR标记
聚类分析
多样性分析
Panicum miliaceum L
Genetic relationship
SSR marker
Cluster analysis
Diversity analysis
