摘要
研究旨在搜寻奶牛HSF1和GRP94基因的SNP位点,找到能提高奶牛产奶性能和乳腺炎抗性的分子遗传标记。以299头中国荷斯坦奶牛为研究对象,采用测序、PCR-RFLP和AS-PCR的方法检测SNP位点,并分析各突变位点基因型与生产性能及乳腺炎抗性指标的相关性。结果表明:在HSF1基因内含子上g.18515T>G、g.18516T>C,在3'-末端g.13809G>T,共2个SNP位点,呈连锁关系;在GRP94基因5'-末端g.-81G>A,内含子上g.13809T>G,共2个SNP位点,也呈连锁关系。关联分析结果显示,HSF1基因SNP位点各基因型与成年当量和乳脂率相关性极显著(P<0.01),联合基因型AA型成年当量极显著高于其他型,但AA型乳脂率极显著低于AB型,可将AA型和AB型分别作为提高奶牛产奶量或乳脂率的候选遗传标记。GRP94基因SNP位点各基因型与体细胞分相关性显著(P<0.05),联合基因型CC型体细胞分极显著低于CD型,可作为提高奶牛乳腺炎抗性的候选遗传标记。
The project was conducted to search SNP loci of dairy HSF1 and GRP94 genes and find potential genetic markers in molecular breeding which could improve milk traits and mastitis resistance. SNP loci were examined at 299 Chinese Holstein dairy cows by sequencing, PCR-RFLP and AS-PCR. The association of genotypes with production traits was analyzed. The results showed that two SNPs (g. 18515T〉 G, g. 18516T〉 C) were found in HSF1 gene's intron and one SNP (g. 13809G〉 T) found in HSF1 gene's 3'-UTR, three SNPs were muta- ted linked. There were also found two SNPs (g. -81G〉A, g. 13809T〉G) located in GRP94 gene's 5'-UTR and intron, respectively. Chi- square testing showed all these loci were not reached Hardy-Weinberg equilibrium. Correlation analysis showed that HSFlgenotypes affected adults milk equivalent weights and fat rates highly significantly (P〈0. 01 ). The adults milk equivalent weights of HSF1 joint genotype AA was highly significantly higher than that of other HSF1 joint genotypes, but fate rates in genotype AA was highly significantly lower than that in genotype AB. HSF1 genotypes of AA or AB could be used as genetic markers to improve milk yield or fat rat, respectively. GRP94 geno- types affected somatic cell score significantly (P〈0. 05 ). The somatic cell score of GRP94 joint genotype CC was highly significantly lower than that of GRP94 joint genotype CD. Together, GRP94 genotype CC could be used as genetic markers to improve mastitis resistance.
出处
《畜牧与兽医》
北大核心
2015年第5期1-6,共6页
Animal Husbandry & Veterinary Medicine
基金
"十二五"国家科技计划课题(2011BAD28B02
2012BAD12B00)