摘要
目的探讨ω-3多不饱和脂肪酸对新生鼠缺血缺氧性脑损伤(HIBD)的保护作用。方法将18只怀孕大鼠随机分为3组,对照组(孕鼠6只,新生鼠26只),假手术组(孕鼠6只,新生鼠24只),干预组(孕鼠6只,新生鼠28只)。干预组,灌胃ω-3多不饱和脂肪酸1 mg·g-1·d-1,从怀孕第2天至分娩后第14天。对照组和假手术组,予等量的0.9%Na Cl溶液。用7 d龄新生鼠制作HIBD模型,用称重法测定脑水肿程度,用Western blot法检测脑组织中诱导型一氧化氮合酶和白细胞介素-6的表达,用改良的Morris水迷宫评估学习与记忆功能。结果干预组,新生鼠脑水肿程度明显轻于对照组(P<0.05);干预组,新生鼠大脑皮层的一氧化氮合酶和白细胞介素-6表达显著高于对照组(P<0.05);干预组,新生鼠学习记忆能力明显强于对照组(P<0.05)。结论ω-3多不饱和脂肪酸可能通过抑制白细胞介素-6、一氧化氮合酶等炎症因子的表达,发挥对新生鼠HIBD的保护作用。
Objective To investigate the protective effect of ω-3 polyunsaturated fatty acid （ω-3 PUFAs ） on hypoxic ischemic brain damage（HIBD） in neonatal rats.Methods Eighteen pregnant rats were randomly divided into 3 groups, control group （6 pregnant rats, 26 new-born rats）, sham operation group （6 pregnant rats, 24 neonatal rats）, intervention group （ 6 pregnant rats , 28 newborn rats ） . Intervention group was administration with ω-3PUFAs （1 mg· g-1· d -1 ） by ga-vage , from 2 d after pregnancy to childbirth after 14 d.The rats in con-trol group and sham operation group were given the same amount of 0.9%NaCl.The newborn mice of 7 d old were made into HIBD model . Brain edema was measured using the gravimetric method .The expression of inducible nitric oxide synthase （ iNOS） and interleukin -6 （ iL-6 ） in brain tissue were detected by Western blot .The learning and memory function was assessed by the modified Morris water maze .Results Com-pared with control group , the newborn mice brain edema degree of inter-vention group was more ease , the difference was statistically significant （P〈0.05）.The expression of iNOS and interleukin -6 in newborn rat cerebral cortex of intervention group was more higher , the difference was statistically significant （ P 〈0.05 ） .The rats of learning and memory;abilityininterventiongroupwasstronger,thedifferencewasstatisticallysignificant（P〈0.05）.Conclusion ω-3 polyunsaturated fatty acids may inhibit the expression of interleukin -6 and iNOS, and play a protective role in HIBD of newborn rat.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2015年第10期865-868,共4页
The Chinese Journal of Clinical Pharmacology
基金
河北省医学科学研究重点课题计划基金资助项目(ZD20140096)
关键词
Ω-3多不饱和脂肪酸
脑缺氧缺血
炎症反应
神经行为功能
ω-3 polyunsaturated fatty acids
hypoxia -ischemia brain damage
inflammatory reaction
neurobehavioral function
